Detailed information of TA system TA076329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2329895..2330568
• Replicon: chromosome
• Accession: NZ_CP021219
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SMRU11_RS30430
• Protein ID: WP_010969926.1
• Coordinates: 2329895..2330299 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0GA10
• Locus tag: SMRU11_RS30435
• Protein ID: WP_003536345.1
• Coordinates: 2330296..2330568 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS30385	2325347..2325613	+	267	WP_010969929.1	hypothetical protein	-
SMRU11_RS30390	2325626..2325940	+	315	WP_003536354.1	antibiotic biosynthesis monooxygenase	-
SMRU11_RS30400	2326223..2326960	-	738	WP_014529841.1	IS21-like element helper ATPase IstB	-
SMRU11_RS30405	2326973..2328502	-	1530	WP_012477342.1	IS21 family transposase	-
SMRU11_RS30415	2328618..2328872	-	255	WP_014529839.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
SMRU11_RS30420	2328995..2329369	-	375	WP_010969927.1	DUF423 domain-containing protein	-
SMRU11_RS30425	2329375..2329767	-	393	WP_003536349.1	globin	-
SMRU11_RS30430	2329895..2330299	-	405	WP_010969926.1	type II toxin-antitoxin system VapC family toxin	Toxin
SMRU11_RS30435	2330296..2330568	-	273	WP_003536345.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SMRU11_RS30440	2330705..2331265	+	561	WP_010969925.1	TetR/AcrR family transcriptional regulator	-
SMRU11_RS30445	2331313..2331654	+	342	WP_003536339.1	EamA family transporter	-
SMRU11_RS30450	2331834..2332889	+	1056	WP_014529838.1	dipeptidase	-
SMRU11_RS30455	2332937..2334577	+	1641	WP_003536336.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14747.98 Da
• Isoelectric Point: 5.1974

>T76329 WP_010969926.1 NZ_CP021219:c2330299-2329895 [Sinorhizobium meliloti RU11/001]
MNGYLLDTNIISDVIHNPFGPAAQRIERIGPKEIYTSIVVASELRYGCAKKGSAKLLAKVESLLEIVPVLPLDIPADTRY
GSIRAELESLGQTIGSNDLLIAAHAYALDLTLVTDNIREFSRVRGLSLENWLER

Nucleotide

• Length: 405 bp

>T76329 NZ_CP021219:c2330299-2329895 [Sinorhizobium meliloti RU11/001]
TTGAACGGCTATCTTCTCGATACGAACATCATCAGCGACGTCATTCACAATCCGTTCGGACCGGCTGCGCAACGCATCGA
ACGGATCGGCCCGAAGGAGATTTACACCAGCATCGTCGTCGCGTCCGAGTTGCGGTATGGTTGCGCAAAAAAGGGATCGG
CGAAGTTGCTCGCCAAGGTCGAAAGCTTGCTGGAAATCGTCCCGGTGCTGCCATTGGATATTCCGGCCGACACCCGATAT
GGCAGCATTCGTGCGGAATTGGAGTCTCTGGGGCAGACCATCGGCTCCAACGATCTGTTGATTGCCGCGCATGCATATGC
GCTGGACCTGACGCTGGTGACGGACAATATCCGTGAGTTCAGCCGCGTTCGTGGCCTTAGTCTGGAAAACTGGCTTGAGC
GATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10334.02 Da
• Isoelectric Point: 6.3950

>AT76329 WP_003536345.1 NZ_CP021219:c2330568-2330296 [Sinorhizobium meliloti RU11/001]
MPVPLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVLIRREGTRLIIEPIARPADIVELLAEWKKEAPLGPEDRFPDVE
DIPARPEKIF

Nucleotide

• Length: 273 bp

>AT76329 NZ_CP021219:c2330568-2330296 [Sinorhizobium meliloti RU11/001]
ATGCCCGTCCCGTTGCCGTCATCGCGACCTAAAGAAGTAAAGCTGTTCCGCAATAACCGCAGCCAGGCAGTCCGTATTCC
TGCGGAGTTCGAGTTGCCTGGAGACCGTGTGTTGATCCGTCGCGAAGGAACGAGACTCATTATCGAACCGATAGCCAGGC
CAGCCGATATCGTGGAGCTTCTCGCGGAGTGGAAGAAGGAAGCTCCCCTCGGACCTGAAGACCGGTTCCCGGATGTTGAG
GATATACCCGCCCGGCCGGAGAAAATCTTTTGA

Structures

Antitoxin

• AlphaFold DB: H0GA10