Detailed information of TA system TA076328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1983046..1983812
• Replicon: chromosome
• Accession: NZ_CP021219
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H0G6D3
• Locus tag: SMRU11_RS28775
• Protein ID: WP_003533488.1
• Coordinates: 1983046..1983528 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H0G6D4
• Locus tag: SMRU11_RS28780
• Protein ID: WP_003533490.1
• Coordinates: 1983528..1983812 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS28745	1978787..1979662	+	876	WP_010970093.1	YihY/virulence factor BrkB family protein	-
SMRU11_RS28750	1979681..1980577	-	897	WP_010970092.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
SMRU11_RS28755	1980730..1981377	+	648	WP_010970091.1	DNA-3-methyladenine glycosylase	-
SMRU11_RS28760	1981483..1982040	+	558	WP_003533486.1	HNH endonuclease	-
SMRU11_RS28765	1982072..1982581	-	510	WP_013844819.1	disulfide bond formation protein B	-
SMRU11_RS28775	1983046..1983528	-	483	WP_003533488.1	GNAT family N-acetyltransferase	Toxin
SMRU11_RS28780	1983528..1983812	-	285	WP_003533490.1	DUF1778 domain-containing protein	Antitoxin
SMRU11_RS28785	1984017..1985663	+	1647	WP_010970088.1	AMP-binding protein	-
SMRU11_RS28790	1985679..1986950	-	1272	WP_010970087.1	GNAT family N-acetyltransferase	-
SMRU11_RS28795	1986954..1988342	-	1389	WP_003533497.1	lipopolysaccharide biosynthesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17463.88 Da
• Isoelectric Point: 7.3827

>T76328 WP_003533488.1 NZ_CP021219:c1983528-1983046 [Sinorhizobium meliloti RU11/001]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Nucleotide

• Length: 483 bp

>T76328 NZ_CP021219:c1983528-1983046 [Sinorhizobium meliloti RU11/001]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10771.12 Da
• Isoelectric Point: 7.3175

>AT76328 WP_003533490.1 NZ_CP021219:c1983812-1983528 [Sinorhizobium meliloti RU11/001]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Nucleotide

• Length: 285 bp

>AT76328 NZ_CP021219:c1983812-1983528 [Sinorhizobium meliloti RU11/001]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Structures

Toxin

• AlphaFold DB: H0G6D3

Antitoxin

• AlphaFold DB: H0G6D4