Detailed information of TA system TA076327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1931545..1932176
• Replicon: chromosome
• Accession: NZ_CP021219
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SMRU11_RS28480
• Protein ID: WP_013844828.1
• Coordinates: 1931778..1932176 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: SMRU11_RS28475
• Protein ID: WP_010970129.1
• Coordinates: 1931545..1931778 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS28455	1927293..1927757	-	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-
SMRU11_RS28460	1927780..1928943	-	1164	WP_010970132.1	amidohydrolase	-
SMRU11_RS28465	1929195..1930277	+	1083	WP_017267062.1	D-alanyl-D-alanine carboxypeptidase	-
SMRU11_RS28470	1930291..1931436	+	1146	WP_010970130.1	GTP-binding protein	-
SMRU11_RS28475	1931545..1931778	+	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SMRU11_RS28480	1931778..1932176	+	399	WP_013844828.1	type II toxin-antitoxin system toxin VapC	Toxin
SMRU11_RS28485	1932210..1932449	-	240	WP_010970127.1	sulfurtransferase TusA family protein	-
SMRU11_RS28490	1932449..1933891	-	1443	WP_014529878.1	murein L,D-transpeptidase	-
SMRU11_RS28495	1934136..1935089	-	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
SMRU11_RS28500	1935153..1936088	-	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
SMRU11_RS28505	1936085..1936234	-	150	WP_010970124.1	hypothetical protein	-
SMRU11_RS28510	1936520..1936795	+	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
SMRU11_RS28515	1936792..1937094	+	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14829.13 Da
• Isoelectric Point: 6.9581

>T76327 WP_013844828.1 NZ_CP021219:1931778-1932176 [Sinorhizobium meliloti RU11/001]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T76327 NZ_CP021219:1931778-1932176 [Sinorhizobium meliloti RU11/001]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCCGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT76327 WP_010970129.1 NZ_CP021219:1931545-1931778 [Sinorhizobium meliloti RU11/001]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT76327 NZ_CP021219:1931545-1931778 [Sinorhizobium meliloti RU11/001]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9