Detailed information of TA system TA076326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1249816..1250477
• Replicon: chromosome
• Accession: NZ_CP021219
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92KY5
• Locus tag: SMRU11_RS25275
• Protein ID: WP_010970565.1
• Coordinates: 1250073..1250477 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92KY4
• Locus tag: SMRU11_RS25270
• Protein ID: WP_010970566.1
• Coordinates: 1249816..1250076 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS25240	1245290..1246171	+	882	WP_010970570.1	pyridoxal kinase PdxY	-
SMRU11_RS25245	1246345..1247028	+	684	WP_010970569.1	carbonic anhydrase	-
SMRU11_RS25255	1247375..1248196	-	822	WP_013844939.1	lytic transglycosylase family protein	-
SMRU11_RS25260	1248369..1249403	-	1035	WP_003531790.1	aspartate-semialdehyde dehydrogenase	-
SMRU11_RS25270	1249816..1250076	+	261	WP_010970566.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SMRU11_RS25275	1250073..1250477	+	405	WP_010970565.1	type II toxin-antitoxin system VapC family toxin	Toxin
SMRU11_RS25280	1250998..1252887	+	1890	WP_014527006.1	MHS family MFS transporter	-
SMRU11_RS25285	1252988..1253695	-	708	WP_010970563.1	phosphatase PAP2 family protein	-
SMRU11_RS25290	1253824..1254936	-	1113	WP_003535893.1	3-isopropylmalate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14507.71 Da
• Isoelectric Point: 6.7257

>T76326 WP_010970565.1 NZ_CP021219:1250073-1250477 [Sinorhizobium meliloti RU11/001]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Nucleotide

• Length: 405 bp

>T76326 NZ_CP021219:1250073-1250477 [Sinorhizobium meliloti RU11/001]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9903.16 Da
• Isoelectric Point: 5.1253

>AT76326 WP_010970566.1 NZ_CP021219:1249816-1250076 [Sinorhizobium meliloti RU11/001]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Nucleotide

• Length: 261 bp

>AT76326 NZ_CP021219:1249816-1250076 [Sinorhizobium meliloti RU11/001]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Structures

Toxin

• AlphaFold DB: Q92KY5

Antitoxin

• AlphaFold DB: Q92KY4