Detailed information of TA system TA076325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 493676..494253
• Replicon: chromosome
• Accession: NZ_CP021219
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: SMRU11_RS21580
• Protein ID: WP_014528972.1
• Coordinates: 493990..494253 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: SMRU11_RS21575
• Protein ID: WP_010968708.1
• Coordinates: 493676..493993 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS21545	489153..489542	+	390	WP_013844021.1	DUF930 domain-containing protein	-
SMRU11_RS21550	489821..490132	-	312	WP_014528976.1	hypothetical protein	-
SMRU11_RS21555	490336..491418	-	1083	WP_014528975.1	nucleotidyltransferase family protein	-
SMRU11_RS21560	491405..492505	-	1101	WP_014528974.1	hypothetical protein	-
SMRU11_RS21565	492824..493012	+	189	WP_017266533.1	hypothetical protein	-
SMRU11_RS21570	493009..493473	+	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
SMRU11_RS21575	493676..493993	-	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
SMRU11_RS21580	493990..494253	-	264	WP_014528972.1	BrnT family toxin	Toxin
SMRU11_RS21590	494838..495050	-	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
SMRU11_RS21595	495055..495675	-	621	WP_014528949.1	Maf-like protein	-
SMRU11_RS21600	495774..495992	-	219	WP_004435948.1	translation initiation factor IF-1	-
SMRU11_RS21605	496150..496629	-	480	WP_010968705.1	low molecular weight phosphatase family protein	-
SMRU11_RS21610	496643..497119	-	477	WP_010968704.1	UPF0262 family protein	-
SMRU11_RS21615	497116..498420	-	1305	WP_014528947.1	histidinol dehydrogenase	-
SMRU11_RS21620	498484..498924	-	441	WP_014528946.1	DUF2948 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T76325 WP_014528972.1 NZ_CP021219:c494253-493990 [Sinorhizobium meliloti RU11/001]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASAIIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T76325 NZ_CP021219:c494253-493990 [Sinorhizobium meliloti RU11/001]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCATCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT76325 WP_010968708.1 NZ_CP021219:c493993-493676 [Sinorhizobium meliloti RU11/001]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT76325 NZ_CP021219:c493993-493676 [Sinorhizobium meliloti RU11/001]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Antitoxin

• AlphaFold DB: Q92KN5