Detailed information of TA system TA076324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 277618..278258
• Replicon: chromosome
• Accession: NZ_CP021219
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H0G6N1
• Locus tag: SMRU11_RS20540
• Protein ID: WP_003533691.1
• Coordinates: 277872..278258 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0G6N0
• Locus tag: SMRU11_RS20535
• Protein ID: WP_003533689.1
• Coordinates: 277618..277872 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS20515	272925..273161	-	237	WP_003533684.1	DUF4170 domain-containing protein	-
SMRU11_RS20520	273222..274040	-	819	WP_010968835.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
SMRU11_RS20525	274149..275495	-	1347	WP_014529073.1	TldD/PmbA family protein	-
SMRU11_RS20530	275647..277479	+	1833	WP_014529072.1	monovalent cation:proton antiporter-2 (CPA2) family protein	-
SMRU11_RS20535	277618..277872	+	255	WP_003533689.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SMRU11_RS20540	277872..278258	+	387	WP_003533691.1	type II toxin-antitoxin system VapC family toxin	Toxin
SMRU11_RS20545	278487..279386	-	900	WP_010968832.1	patatin-like phospholipase family protein	-
SMRU11_RS20550	279459..280019	-	561	WP_010968831.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
SMRU11_RS20555	280026..281747	-	1722	WP_010968830.1	pseudouridine synthase	-
SMRU11_RS20560	281822..282271	+	450	WP_003537305.1	nucleoside deaminase	-
SMRU11_RS20565	282315..282794	-	480	WP_003537307.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13904.77 Da
• Isoelectric Point: 4.5716

>T76324 WP_003533691.1 NZ_CP021219:277872-278258 [Sinorhizobium meliloti RU11/001]
MVIDTSAIAAIAFNEQEAGSFREKIADDPVRLISAATALEAAMVIETRLGEAAGAELDLWLYKANVEIVAVTAEHMDQAR
RAWRRFGKGRHPAGLNFGDCFSYALAFLTNEPLLFKGSDFSQTDIPAA

Nucleotide

• Length: 387 bp

>T76324 NZ_CP021219:277872-278258 [Sinorhizobium meliloti RU11/001]
ATGGTCATCGACACATCCGCCATTGCCGCGATTGCCTTCAATGAACAGGAGGCCGGAAGTTTCCGCGAAAAAATCGCGGA
CGACCCGGTTCGGCTCATCTCCGCGGCAACCGCTCTGGAAGCGGCCATGGTAATCGAAACCCGGTTAGGCGAAGCTGCCG
GAGCGGAACTTGATCTTTGGCTTTACAAGGCAAACGTGGAGATTGTTGCGGTAACCGCCGAGCATATGGACCAGGCCCGC
CGTGCCTGGCGCCGTTTCGGCAAAGGCCGTCATCCGGCCGGTTTGAATTTCGGTGATTGTTTTTCCTATGCCCTGGCCTT
TCTGACAAATGAACCGCTTCTCTTCAAGGGAAGCGATTTCTCGCAAACCGATATCCCGGCGGCATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9472.63 Da
• Isoelectric Point: 4.8660

>AT76324 WP_003533689.1 NZ_CP021219:277618-277872 [Sinorhizobium meliloti RU11/001]
MALSIKDTETEQLARTLARRTGESITLATKRALEERLRRTDTAARRAALLEDLEAIQRRWNAMPVLDSRSSDDIIGYDED
GLPS

Nucleotide

• Length: 255 bp

>AT76324 NZ_CP021219:277618-277872 [Sinorhizobium meliloti RU11/001]
ATGGCGCTGAGCATCAAGGACACTGAAACCGAGCAGCTTGCCCGCACACTTGCCCGGCGGACAGGGGAATCCATCACGCT
TGCCACCAAGCGTGCCCTCGAAGAACGATTGCGCCGAACCGACACCGCTGCCCGGCGCGCGGCTCTTCTGGAGGATCTGG
AAGCCATTCAACGCCGCTGGAATGCCATGCCCGTCCTCGACAGTCGCAGCTCCGATGACATTATCGGTTATGACGAGGAT
GGACTCCCCTCATAA

Structures

Toxin

• AlphaFold DB: H0G6N1

Antitoxin

• AlphaFold DB: H0G6N0