Detailed information of TA system TA076320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 140536..141070
• Replicon: plasmid pSymB
• Accession: NZ_CP021218
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q92X99
• Locus tag: SMRU11_RS11690
• Protein ID: WP_010974986.1
• Coordinates: 140536..140832 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: SMRU11_RS11695
• Protein ID: WP_003527850.1
• Coordinates: 140822..141070 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS11660	135978..137381	-	1404	WP_026029937.1	sodium:alanine symporter family protein	-
SMRU11_RS11665	137915..138124	-	210	WP_003527838.1	dodecin family protein	-
SMRU11_RS11670	138414..138860	+	447	WP_014527651.1	DNA-binding protein	-
SMRU11_RS11675	138864..139049	-	186	WP_010974989.1	hypothetical protein	-
SMRU11_RS11680	139216..139614	-	399	WP_010974988.1	hypothetical protein	-
SMRU11_RS11685	140195..140455	+	261	Protein_133	helix-turn-helix domain containing protein	-
SMRU11_RS11690	140536..140832	-	297	WP_010974986.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SMRU11_RS11695	140822..141070	-	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
SMRU11_RS11710	141688..141813	+	126	Protein_136	IS481 family transposase	-
SMRU11_RS11715	141897..142691	-	795	WP_013850206.1	methyltransferase domain-containing protein	-
SMRU11_RS11720	142695..143495	-	801	WP_015008735.1	ABC transporter ATP-binding protein	-
SMRU11_RS11725	143492..144535	-	1044	WP_010974980.1	iron ABC transporter permease	-
SMRU11_RS11730	144532..145599	-	1068	WP_017266564.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1686813	1686813
	-	flank	IS/Tn	-	-	141622..141813	191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-91)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11473.15 Da
• Isoelectric Point: 10.6593

>T76320 WP_010974986.1 NZ_CP021218:c140832-140536 [Sinorhizobium meliloti RU11/001]
MATERPFRLAPTAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAVGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRVDAQRHL

Nucleotide

• Length: 297 bp

>T76320 NZ_CP021218:c140832-140536 [Sinorhizobium meliloti RU11/001]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTACCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGTAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGAATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT76320 WP_003527850.1 NZ_CP021218:c141070-140822 [Sinorhizobium meliloti RU11/001]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT76320 NZ_CP021218:c141070-140822 [Sinorhizobium meliloti RU11/001]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CCTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Toxin

• AlphaFold DB: Q92X99

Antitoxin

• AlphaFold DB: H0FXK0