76319

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 984072..984652 Replicon plasmid pSymA
Accession NZ_CP021217
Organism Sinorhizobium meliloti RU11/001

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag SMRU11_RS07470 Protein ID WP_010968151.1
Coordinates 984269..984652 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag SMRU11_RS07465 Protein ID WP_013851145.1
Coordinates 984072..984272 (+) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SMRU11_RS07440 981041..981187 - 147 WP_017266778.1 hypothetical protein -
SMRU11_RS07445 981281..982009 - 729 WP_014531163.1 class I SAM-dependent methyltransferase -
SMRU11_RS07450 982048..982371 - 324 WP_017266777.1 DUF1127 domain-containing protein -
SMRU11_RS07455 982467..983303 + 837 WP_014531162.1 helix-turn-helix transcriptional regulator -
SMRU11_RS07460 983380..983781 - 402 WP_014531161.1 GFA family protein -
SMRU11_RS07465 984072..984272 + 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
SMRU11_RS07470 984269..984652 + 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
SMRU11_RS07475 984998..985258 - 261 WP_013845025.1 hypothetical protein -
SMRU11_RS07480 985452..985658 + 207 WP_013845026.1 CsbD family protein -
SMRU11_RS07485 986704..986979 - 276 WP_014531159.1 hypothetical protein -
SMRU11_RS07495 987541..987951 + 411 WP_014531156.1 hypothetical protein -
SMRU11_RS07500 988327..989442 - 1116 WP_017271826.1 AI-2E family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB / htpB 1..1613292 1613292


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T76319 WP_010968151.1 NZ_CP021217:984269-984652 [Sinorhizobium meliloti RU11/001]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T76319 NZ_CP021217:984269-984652 [Sinorhizobium meliloti RU11/001]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT76319 WP_013851145.1 NZ_CP021217:984072-984272 [Sinorhizobium meliloti RU11/001]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT76319 NZ_CP021217:984072-984272 [Sinorhizobium meliloti RU11/001]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References