Detailed information of TA system TA076318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 941354..941949
• Replicon: plasmid pSymA
• Accession: NZ_CP021217
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F7XBJ0
• Locus tag: SMRU11_RS07190
• Protein ID: WP_014531932.1
• Coordinates: 941354..941644 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: SMRU11_RS07195
• Protein ID: WP_010967242.1
• Coordinates: 941641..941949 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS07170	936929..937828	+	900	WP_010967198.1	LysR family transcriptional regulator	-
SMRU11_RS07175	938270..939270	+	1001	Protein_888	IS30 family transposase	-
SMRU11_RS07180	939362..939619	-	258	WP_080567622.1	hypothetical protein	-
SMRU11_RS07185	939893..941092	+	1200	WP_014531931.1	NAD-dependent formate dehydrogenase	-
SMRU11_RS07190	941354..941644	-	291	WP_014531932.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
SMRU11_RS07195	941641..941949	-	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SMRU11_RS07200	942294..942506	+	213	WP_013845891.1	hypothetical protein	-
SMRU11_RS07205	942658..942924	-	267	WP_010967197.1	EAL domain-containing protein	-
SMRU11_RS07215	943964..945748	-	1785	WP_014531184.1	polysaccharide biosynthesis/export family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1613292	1613292
	-	flank	IS/Tn	-	-	938896..939270	374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11195.84 Da
• Isoelectric Point: 6.4734

>T76318 WP_014531932.1 NZ_CP021217:c941644-941354 [Sinorhizobium meliloti RU11/001]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAATRELVIPRTYIAAYMVMEDRIRIL
RVLHGAQKWPSELDDG

Nucleotide

• Length: 291 bp

>T76318 NZ_CP021217:c941644-941354 [Sinorhizobium meliloti RU11/001]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGCAACTCGAGAGCTTGTTATCCCTCGGACCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATTCTG
CGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT76318 WP_010967242.1 NZ_CP021217:c941949-941641 [Sinorhizobium meliloti RU11/001]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT76318 NZ_CP021217:c941949-941641 [Sinorhizobium meliloti RU11/001]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: F7XBJ0

Antitoxin

• AlphaFold DB: Q930F1