Detailed information of TA system TA076317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 494567..495252
• Replicon: plasmid pSymA
• Accession: NZ_CP021217
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SMRU11_RS04810
• Protein ID: WP_014531572.1
• Coordinates: 494833..495252 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: SMRU11_RS04805
• Protein ID: WP_013845417.1
• Coordinates: 494567..494836 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS04770	489616..489975	-	360	Protein_460	FAD:protein FMN transferase	-
SMRU11_RS04775	490085..490954	-	870	WP_014528623.1	aldo/keto reductase family oxidoreductase	-
SMRU11_RS04780	491071..491994	+	924	WP_013845420.1	LysR family transcriptional regulator	-
SMRU11_RS04795	492974..493849	+	876	WP_014531570.1	LysR family transcriptional regulator	-
SMRU11_RS04800	493937..494456	-	520	Protein_464	hypothetical protein	-
SMRU11_RS04805	494567..494836	+	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SMRU11_RS04810	494833..495252	+	420	WP_014531572.1	type II toxin-antitoxin system VapC family toxin	Toxin
SMRU11_RS04815	495406..495819	-	414	WP_014531574.1	hypothetical protein	-
SMRU11_RS04820	496089..496400	+	312	WP_017266244.1	type II toxin-antitoxin system HigB family toxin	-
SMRU11_RS04825	496418..496798	+	381	WP_003526626.1	transcriptional regulator	-
SMRU11_RS04830	497058..498059	+	1002	WP_014531093.1	IS30 family transposase	-
SMRU11_RS04835	498185..499558	+	1374	WP_014531577.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1613292	1613292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14778.09 Da
• Isoelectric Point: 4.8721

>T76317 WP_014531572.1 NZ_CP021217:494833-495252 [Sinorhizobium meliloti RU11/001]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSC

Nucleotide

• Length: 420 bp

>T76317 NZ_CP021217:494833-495252 [Sinorhizobium meliloti RU11/001]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGTGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT76317 WP_013845417.1 NZ_CP021217:494567-494836 [Sinorhizobium meliloti RU11/001]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT76317 NZ_CP021217:494567-494836 [Sinorhizobium meliloti RU11/001]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTTCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCCGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5