Detailed information of TA system TA076316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 163366..163865
• Replicon: plasmid pSymA
• Accession: NZ_CP021217
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q92YA6
• Locus tag: SMRU11_RS03160
• Protein ID: WP_010967939.1
• Coordinates: 163692..163865 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: SMRU11_RS40060
• Protein ID: WP_017272263.1
• Coordinates: 163366..163695 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS03130	159043..159936	-	894	WP_010967943.1	LysR family transcriptional regulator	-
SMRU11_RS03135	160028..160471	+	444	WP_010967942.1	PACE efflux transporter	-
SMRU11_RS03140	160588..160926	+	339	WP_013845641.1	SDR family oxidoreductase	-
SMRU11_RS03145	160910..161251	-	342	Protein_154	hypothetical protein	-
SMRU11_RS03150	161635..163005	-	1371	WP_017265792.1	alpha/beta hydrolase	-
SMRU11_RS40060	163366..163695	+	330	WP_017272263.1	hypothetical protein	Antitoxin
SMRU11_RS03160	163692..163865	+	174	WP_010967939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SMRU11_RS03165	163963..164268	-	306	WP_013845638.1	hypothetical protein	-
SMRU11_RS03170	164354..164593	-	240	WP_017272264.1	hypothetical protein	-
SMRU11_RS03175	164586..164945	-	360	WP_013845635.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
SMRU11_RS03180	165540..165791	+	252	WP_003534243.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
SMRU11_RS03185	165819..166304	+	486	WP_014531392.1	YidB family protein	-
SMRU11_RS03190	166674..167990	-	1317	WP_014531394.1	aspartate aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1613292	1613292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6497.32 Da
• Isoelectric Point: 5.7733

>T76316 WP_010967939.1 NZ_CP021217:163692-163865 [Sinorhizobium meliloti RU11/001]
MKLVWARYALDDRDAIFSYIERENPRAAVHVDEEVVSAGRPLDFPESRRPGRIAGTP

Nucleotide

• Length: 174 bp

>T76316 NZ_CP021217:163692-163865 [Sinorhizobium meliloti RU11/001]
ATGAAGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACGCCATCTTCAGCTACATCGAACGAGAGAATCCGAGGGC
GGCGGTCCACGTTGACGAGGAGGTCGTCAGCGCTGGTCGTCCGCTGGACTTTCCCGAAAGCCGCCGCCCTGGCCGGATTG
CCGGAACTCCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12025.64 Da
• Isoelectric Point: 11.1401

>AT76316 WP_017272263.1 NZ_CP021217:163366-163695 [Sinorhizobium meliloti RU11/001]
VSERLLLIALLALGPFRASQRHTGRPALLRRGPQSAAGAVEEHFIQLRAPLWLITLGGFSHREAHDAWFRAKALRALEDT
RPDADDADADAHFQERRAAALRKAAAGDR

Nucleotide

• Length: 330 bp

>AT76316 NZ_CP021217:163366-163695 [Sinorhizobium meliloti RU11/001]
GTGAGCGAGCGCCTTCTGCTCATCGCCCTCCTGGCCCTCGGTCCGTTCCGAGCCAGCCAGCGGCATACCGGTCGTCCTGC
TCTGCTACGGCGTGGCCCCCAATCGGCCGCCGGCGCGGTCGAGGAGCACTTCATCCAACTCCGCGCTCCGCTGTGGCTGA
TCACGCTCGGCGGTTTCAGCCACAGAGAGGCTCACGACGCTTGGTTCCGCGCCAAAGCTTTGCGGGCACTCGAGGACACT
CGACCCGATGCGGACGACGCCGACGCCGATGCGCACTTTCAAGAGCGCCGCGCCGCAGCTTTGCGCAAGGCTGCGGCGGG
TGATCGATGA

Structures

Toxin

• AlphaFold DB: Q92YA6