Detailed information of TA system TA076315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 120705..121348
• Replicon: plasmid pSmeRU11c
• Accession: NZ_CP021215
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SMRU11_RS01385
• Protein ID: WP_012881218.1
• Coordinates: 120962..121348 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1WLI3
• Locus tag: SMRU11_RS01380
• Protein ID: WP_012881219.1
• Coordinates: 120705..120962 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS40580	115736..116500	-	765	WP_049223499.1	glycosyl hydrolase	-
SMRU11_RS40585	116474..117526	-	1053	Protein_114	peptidase M16	-
SMRU11_RS01360	117671..118651	-	981	WP_012881223.1	IS5 family transposase	-
SMRU11_RS01370	119157..120146	+	990	WP_012881221.1	IS5-like element ISRm33 family transposase	-
SMRU11_RS01375	120174..120536	-	363	WP_017265936.1	DUF1403 family protein	-
SMRU11_RS01380	120705..120962	+	258	WP_012881219.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SMRU11_RS01385	120962..121348	+	387	WP_012881218.1	type II toxin-antitoxin system VapC family toxin	Toxin
SMRU11_RS01390	121348..122397	+	1050	WP_012881217.1	site-specific integrase	-
SMRU11_RS01395	122489..125869	+	3381	WP_012881216.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..144210	144210
	-	flank	IS/Tn	-	-	119295..120146	851

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (2-84)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14030.28 Da
• Isoelectric Point: 4.5849

>T76315 WP_012881218.1 NZ_CP021215:120962-121348 [Sinorhizobium meliloti RU11/001]
MVIDTSAIVAILRNEPEAAKLERALVANPVRLVPATCVLEARMVLVSRRGEHALAEIDLWLSKIEADIISVDSDLVDLAT
QAWLIYGKGRHPAALNFADCFSYALAKRADEPLLFIGKDFLQTDIEAA

Nucleotide

• Length: 387 bp

>T76315 NZ_CP021215:120962-121348 [Sinorhizobium meliloti RU11/001]
ATGGTGATCGATACCTCCGCCATCGTGGCGATTCTGCGTAACGAGCCGGAAGCGGCCAAGCTCGAAAGGGCGCTCGTCGC
CAACCCAGTTCGGCTTGTCCCTGCGACGTGTGTGCTCGAGGCGCGCATGGTGCTGGTCAGCCGGCGCGGAGAACACGCAC
TGGCCGAAATCGATCTTTGGCTTAGCAAAATCGAGGCTGACATCATCTCTGTCGATTCTGATCTGGTCGATTTGGCGACG
CAGGCCTGGCTGATCTACGGCAAGGGCCGCCATCCGGCGGCTTTGAATTTTGCCGACTGCTTTTCCTACGCTTTGGCAAA
GCGTGCGGACGAACCGCTTCTCTTCATCGGCAAGGACTTTTTGCAAACCGATATCGAGGCGGCGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9474.69 Da
• Isoelectric Point: 6.8806

>AT76315 WP_012881219.1 NZ_CP021215:120705-120962 [Sinorhizobium meliloti RU11/001]
VSLNIKNPATVALADELARRQGISKTAAIHQALSERLHRMGHGEVSQDRLLGELRAIRQRIARLPELDSRSDEEIIGYDE
HGIPN

Nucleotide

• Length: 258 bp

>AT76315 NZ_CP021215:120705-120962 [Sinorhizobium meliloti RU11/001]
GTGTCGCTGAACATCAAAAATCCAGCCACTGTAGCGCTCGCTGACGAACTGGCGCGCCGCCAAGGAATCAGCAAGACGGC
AGCCATCCATCAGGCCCTAAGCGAACGTCTCCACCGTATGGGTCACGGGGAGGTCTCCCAGGATCGTCTGCTCGGCGAAT
TACGCGCTATCCGGCAAAGGATAGCACGTCTTCCGGAATTGGACAGTCGCAGTGACGAGGAGATCATCGGATATGACGAG
CATGGAATTCCGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A2Z4YSJ3