Detailed information of TA system TA076314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 61525..62162
• Replicon: plasmid pSmeRU11b
• Accession: NZ_CP021214
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SMRU11_RS00625
• Protein ID: WP_029728330.1
• Coordinates: 61525..61926 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SMRU11_RS00630
• Protein ID: WP_029728331.1
• Coordinates: 61926..62162 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS39980	58541..58741	+	201	WP_158529805.1	hypothetical protein	-
SMRU11_RS00610	58990..60011	-	1022	Protein_59	IS110 family transposase	-
SMRU11_RS00615	60100..60561	-	462	Protein_60	hypothetical protein	-
SMRU11_RS00625	61525..61926	-	402	WP_029728330.1	type II toxin-antitoxin system VapC family toxin	Toxin
SMRU11_RS00630	61926..62162	-	237	WP_029728331.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SMRU11_RS00635	62747..63214	-	468	Protein_63	Tn3 family transposase	-
SMRU11_RS00640	63389..64897	+	1509	WP_088194112.1	IS21-like element ISRm9 family transposase	-
SMRU11_RS00645	64894..65707	+	814	Protein_65	IS21-like element ISRm9 family helper ATPase IstB	-
SMRU11_RS00650	66000..66205	-	206	Protein_66	Tn3 family transposase	-
SMRU11_RS00655	66398..67081	-	684	WP_017273848.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86642	86642

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-117)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14832.08 Da
• Isoelectric Point: 5.1659

>T76314 WP_029728330.1 NZ_CP021214:c61926-61525 [Sinorhizobium meliloti RU11/001]
MARYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELEYGVSASDDPARELDNLAALAELITVEPFGIAAARA
YGPVRMATRDRKKDHLDKLIASHAIALNTVLVTNNTRDFLVYTGLKLENWLDA

Nucleotide

• Length: 402 bp

>T76314 NZ_CP021214:c61926-61525 [Sinorhizobium meliloti RU11/001]
ATGGCGCGCTACATGCTCGATACGAATATGTGCATCTACCTGATGAAAAACCAGCCGGAGCAGGTCGCCCGCCGCTTTGC
CAGTTGCTATGTGGGAGACGTGGTCATGTCCGCCATCACCTTCGCCGAACTCGAGTATGGTGTATCCGCATCCGATGATC
CCGCACGGGAACTTGATAATCTCGCAGCCCTGGCCGAGCTGATCACAGTGGAACCGTTTGGGATTGCCGCAGCGAGAGCC
TATGGGCCGGTTCGGATGGCGACAAGAGACCGGAAGAAAGATCATCTCGATAAACTGATCGCGTCGCATGCGATCGCACT
TAACACGGTGTTGGTGACCAATAACACGCGCGACTTCCTGGTGTATACCGGACTGAAGCTCGAAAACTGGCTGGACGCGT
AA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8951.03 Da
• Isoelectric Point: 6.2256

>AT76314 WP_029728331.1 NZ_CP021214:c62162-61926 [Sinorhizobium meliloti RU11/001]
MHTTKAFKNGNSQAVRIPAELAFDRTDVELEIERVGDELRIRPVHRSLAGVLDVFARFSPDFMRDGRGSHEQDERDKI

Nucleotide

• Length: 237 bp

>AT76314 NZ_CP021214:c62162-61926 [Sinorhizobium meliloti RU11/001]
ATGCACACCACGAAAGCTTTCAAAAACGGAAATTCGCAGGCCGTGCGTATCCCGGCCGAGCTTGCTTTCGACCGGACCGA
TGTCGAGTTGGAAATCGAGCGCGTCGGCGACGAGCTTCGTATCCGTCCTGTGCATCGCTCCCTGGCCGGCGTTCTGGATG
TATTCGCCCGCTTCTCTCCGGATTTTATGCGTGACGGAAGAGGGTCCCATGAGCAAGACGAGCGGGATAAGATCTGA