Detailed information of TA system TA076313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RHH
• Location: 43310..43863
• Replicon: plasmid pSmeRU11a
• Accession: NZ_CP021213
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: SMRU11_RS00220
• Protein ID: WP_029728301.1
• Coordinates: 43310..43603 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: SMRU11_RS00225
• Protein ID: WP_013845962.1
• Coordinates: 43591..43863 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS00195	38649..38861	-	213	WP_029728305.1	ribbon-helix-helix protein, CopG family	-
SMRU11_RS00200	39229..41246	-	2018	Protein_40	ParB/RepB/Spo0J family partition protein	-
SMRU11_RS00205	41316..41895	-	580	Protein_41	hypothetical protein	-
SMRU11_RS00210	41892..42089	-	198	WP_013845954.1	hypothetical protein	-
SMRU11_RS00215	42547..43083	+	537	WP_029728303.1	hypothetical protein	-
SMRU11_RS00220	43310..43603	-	294	WP_029728301.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SMRU11_RS00225	43591..43863	-	273	WP_013845962.1	CopG family ribbon-helix-helix protein	Antitoxin
SMRU11_RS00230	44072..44301	-	230	Protein_46	hypothetical protein	-
SMRU11_RS00235	44571..44906	+	336	WP_080654722.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SMRU11_RS00240	45050..45265	-	216	WP_080654721.1	hypothetical protein	-
SMRU11_RS00245	45315..45620	-	306	WP_029728298.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SMRU11_RS00250	45617..45892	-	276	WP_029728296.1	type II toxin-antitoxin system ParD family antitoxin	-
SMRU11_RS00255	46180..46809	+	630	WP_049223453.1	ParA family protein	-
SMRU11_RS00260	46809..47105	+	297	WP_080654720.1	hypothetical protein	-
SMRU11_RS00270	47315..48493	+	1179	Protein_53	IS21-like element ISRm9 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56395	56395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10767.23 Da
• Isoelectric Point: 5.1693

>T76313 WP_029728301.1 NZ_CP021213:c43603-43310 [Sinorhizobium meliloti RU11/001]
VPQVIVTEGAAEGLERCRRFLATKAPEAARRAGQAIERQFLLLETAPDIGRPFPEAPELRELVIAFGDSGYVALYRHEPA
DDAVYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T76313 NZ_CP021213:c43603-43310 [Sinorhizobium meliloti RU11/001]
GTGCCACAAGTAATCGTCACCGAAGGCGCTGCGGAAGGCTTGGAGCGATGCCGGCGCTTCCTGGCCACTAAGGCCCCGGA
AGCCGCCAGACGGGCCGGCCAGGCTATCGAGCGGCAATTCCTGCTTCTGGAGACGGCGCCCGACATTGGCCGGCCGTTCC
CCGAAGCGCCTGAGCTGCGCGAGCTGGTGATTGCCTTCGGGGACTCCGGCTATGTGGCCCTGTACCGTCACGAGCCGGCC
GACGATGCGGTGTATGTTCTGGCCTTCCGGCACCAGAAAGAAGCGGGCTATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10561.83 Da
• Isoelectric Point: 6.7205

>AT76313 WP_013845962.1 NZ_CP021213:c43863-43591 [Sinorhizobium meliloti RU11/001]
MATSLKIDDTLKSRVQQLASQRRRSSHWIMLEAIQQYVEREEARESFKQEALASWAAYKETGRHLTGEEVRTWLTTWGTD
DEKPVPECHK

Nucleotide

• Length: 273 bp

>AT76313 NZ_CP021213:c43863-43591 [Sinorhizobium meliloti RU11/001]
ATGGCAACGTCTCTCAAGATCGACGACACACTGAAAAGTCGCGTCCAGCAGCTCGCCAGCCAGCGCCGCCGCTCGTCTCA
CTGGATCATGCTCGAAGCGATCCAACAGTACGTTGAGCGCGAGGAAGCACGCGAGAGCTTCAAGCAGGAGGCCTTGGCGT
CCTGGGCCGCGTACAAGGAAACCGGTCGCCACCTGACCGGCGAAGAAGTCCGCACCTGGTTGACTACCTGGGGTACCGAT
GACGAAAAGCCGGTGCCAGAGTGCCACAAGTAA