Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4492600..4493218 Replicon chromosome
Accession NZ_CP021207
Organism Escherichia coli strain strain Z247

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CA270_RS23060 Protein ID WP_001291435.1
Coordinates 4492600..4492818 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CA270_RS23065 Protein ID WP_000344800.1
Coordinates 4492844..4493218 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA270_RS23025 4487887..4488459 + 573 WP_000779831.1 YbaY family lipoprotein -
CA270_RS23030 4488490..4488801 - 312 WP_000409908.1 MGMT family protein -
CA270_RS23040 4489180..4489533 + 354 WP_000878135.1 DUF1428 family protein -
CA270_RS23045 4489575..4491125 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CA270_RS23050 4491289..4491759 - 471 WP_000136192.1 YlaC family protein -
CA270_RS23055 4491875..4492426 - 552 WP_000102539.1 maltose O-acetyltransferase -
CA270_RS23060 4492600..4492818 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CA270_RS23065 4492844..4493218 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CA270_RS23070 4493764..4496913 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
CA270_RS23075 4496936..4498129 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T76302 WP_001291435.1 NZ_CP021207:c4492818-4492600 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T76302 NZ_CP021207:c4492818-4492600 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT76302 WP_000344800.1 NZ_CP021207:c4493218-4492844 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT76302 NZ_CP021207:c4493218-4492844 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References