Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4030615..4031135 Replicon chromosome
Accession NZ_CP021207
Organism Escherichia coli strain strain Z247

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag CA270_RS20705 Protein ID WP_001160964.1
Coordinates 4030851..4031135 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CA270_RS20700 Protein ID WP_000125566.1
Coordinates 4030615..4030848 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA270_RS20675 4025688..4025975 + 288 WP_000203744.1 ferredoxin-like protein FixX -
CA270_RS20680 4026034..4027365 + 1332 WP_001183209.1 MFS transporter -
CA270_RS20685 4027473..4028003 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CA270_RS20690 4027996..4029858 + 1863 WP_061089100.1 glutathione-regulated potassium-efflux system protein KefC -
CA270_RS20695 4030050..4030529 + 480 WP_024190995.1 type 3 dihydrofolate reductase -
CA270_RS20700 4030615..4030848 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CA270_RS20705 4030851..4031135 + 285 WP_001160964.1 CcdB family protein Toxin
CA270_RS20710 4031162..4032010 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CA270_RS20715 4032017..4032394 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CA270_RS20720 4032397..4033218 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CA270_RS20725 4033215..4034204 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CA270_RS20730 4034204..4035490 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T76300 WP_001160964.1 NZ_CP021207:4030851-4031135 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T76300 NZ_CP021207:4030851-4031135 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACTAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT76300 WP_000125566.1 NZ_CP021207:4030615-4030848 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT76300 NZ_CP021207:4030615-4030848 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References