Detailed information of TA system TA076271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 51493..52015
• Replicon: plasmid p1002-MCR1
• Accession: NZ_CP021205
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: CA268_RS28905
• Protein ID: WP_000638823.1
• Coordinates: 51734..52015 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: CA268_RS28900
• Protein ID: WP_000121741.1
• Coordinates: 51493..51744 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS28850	46582..47025	+	444	WP_000964330.1	NfeD family protein	-
CA268_RS30745	47029..47199	+	171	WP_000550720.1	hypothetical protein	-
CA268_RS28855	47210..47656	+	447	WP_001243165.1	hypothetical protein	-
CA268_RS28860	47689..47946	+	258	WP_001542015.1	hypothetical protein	-
CA268_RS28865	47927..48229	+	303	WP_001360345.1	hypothetical protein	-
CA268_RS28870	48226..48483	+	258	WP_000739144.1	hypothetical protein	-
CA268_RS28875	48487..49482	+	996	WP_001028543.1	type IV secretion system protein	-
CA268_RS28880	49488..50129	+	642	WP_001425343.1	conjugal transfer protein TrbJ	-
CA268_RS28885	50131..50385	+	255	WP_001043555.1	EexN family lipoprotein	-
CA268_RS28890	50398..50685	+	288	WP_001032611.1	EexN family lipoprotein	-
CA268_RS28895	50758..51393	+	636	WP_015059536.1	hypothetical protein	-
CA268_RS28900	51493..51744	+	252	WP_000121741.1	hypothetical protein	Antitoxin
CA268_RS28905	51734..52015	+	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA268_RS28910	52083..52382	+	300	WP_000835764.1	IncN plasmid KikA protein	-
CA268_RS28915	52385..53620	+	1236	WP_015059538.1	TcpQ domain-containing protein	-
CA268_RS28920	53626..54063	+	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
CA268_RS28925	54182..54580	+	399	WP_001153665.1	hypothetical protein	-
CA268_RS28930	54601..55185	+	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
CA268_RS28935	55287..55475	+	189	WP_050567133.1	hypothetical protein	-
CA268_RS28940	55546..55866	+	321	WP_000362080.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..63392	63392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T76271 WP_000638823.1 NZ_CP021205:51734-52015 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T76271 NZ_CP021205:51734-52015 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT76271 WP_000121741.1 NZ_CP021205:51493-51744 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT76271 NZ_CP021205:51493-51744 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1