Detailed information of TA system TA076270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 34039..34540
• Replicon: plasmid p1002-MCR1
• Accession: NZ_CP021205
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: CA268_RS28740
• Protein ID: WP_000702247.1
• Coordinates: 34280..34540 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: CA268_RS28735
• Protein ID: WP_001132407.1
• Coordinates: 34039..34290 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS28685	29096..29848	+	753	WP_063078685.1	conjugal transfer protein TraL	-
CA268_RS28690	29850..30557	+	708	WP_021503228.1	hypothetical protein	-
CA268_RS28695	30577..30903	+	327	WP_063078684.1	hypothetical protein	-
CA268_RS28700	31034..31471	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
CA268_RS28705	31496..31678	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
CA268_RS28710	32236..32418	+	183	WP_001481829.1	hypothetical protein	-
CA268_RS28715	32525..32791	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
CA268_RS28720	33211..33489	-	279	WP_000545938.1	hypothetical protein	-
CA268_RS28735	34039..34290	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
CA268_RS28740	34280..34540	-	261	WP_000702247.1	hypothetical protein	Toxin
CA268_RS28745	34592..34816	-	225	WP_000814381.1	hypothetical protein	-
CA268_RS28750	34913..35122	-	210	WP_000866036.1	hypothetical protein	-
CA268_RS28755	35164..35487	-	324	WP_000427403.1	hypothetical protein	-
CA268_RS28765	36069..36398	-	330	WP_000855120.1	hypothetical protein	-
CA268_RS28775	36996..37661	+	666	WP_000532148.1	P-loop NTPase	-
CA268_RS30825	37735..38019	+	285	WP_001162371.1	hypothetical protein	-
CA268_RS28785	38037..38633	-	597	WP_000125165.1	hypothetical protein	-
CA268_RS28790	38854..39042	+	189	WP_000957857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..63392	63392
	-	flank	IS/Tn	-	-	39052..40251	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T76270 WP_000702247.1 NZ_CP021205:c34540-34280 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T76270 NZ_CP021205:c34540-34280 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT76270 WP_001132407.1 NZ_CP021205:c34290-34039 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT76270 NZ_CP021205:c34290-34039 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0