Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 76811..77370 Replicon plasmid p1002-4
Accession NZ_CP021204
Organism Escherichia coli strain Z1002

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag CA268_RS28415 Protein ID WP_000421272.1
Coordinates 77095..77370 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CA268_RS28410 Protein ID WP_001387467.1
Coordinates 76811..77095 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA268_RS28380 72393..73097 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
CA268_RS28390 73133..74005 + 873 WP_085693684.1 dihydrodipicolinate synthase family protein -
CA268_RS28395 74030..74410 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
CA268_RS28400 74443..75408 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
CA268_RS28405 75454..76206 - 753 WP_000562172.1 IclR family transcriptional regulator -
CA268_RS28410 76811..77095 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CA268_RS28415 77095..77370 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CA268_RS28420 77486..78183 - 698 Protein_95 IS1 family transposase -
CA268_RS28430 78253..78546 + 294 WP_001105060.1 hypothetical protein -
CA268_RS30430 78581..78709 - 129 Protein_97 IS481 family transposase -
CA268_RS28435 78742..79911 - 1170 WP_001072355.1 hypothetical protein -
CA268_RS28445 80404..80711 + 308 Protein_99 type II toxin-antitoxin system ParD family antitoxin -
CA268_RS30435 81054..81236 + 183 WP_042344623.1 hypothetical protein -
CA268_RS28460 81357..82097 + 741 WP_001066953.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B - 1..92438 92438
- inside IScluster/Tn blaTEM-1B - 70523..78183 7660


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T76269 WP_000421272.1 NZ_CP021204:77095-77370 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T76269 NZ_CP021204:77095-77370 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT76269 WP_001387467.1 NZ_CP021204:76811-77095 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT76269 NZ_CP021204:76811-77095 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References