76260

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 53445..54046 Replicon plasmid p1002-1
Accession NZ_CP021203
Organism Escherichia coli strain Z1002

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag CA268_RS27020 Protein ID WP_001216045.1
Coordinates 53445..53825 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CA268_RS27025 Protein ID WP_001190712.1
Coordinates 53825..54046 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA268_RS26995 48886..50370 - 1485 WP_000124159.1 hypothetical protein -
CA268_RS27000 50370..51563 - 1194 WP_060578234.1 hypothetical protein -
CA268_RS27005 51649..52101 - 453 WP_001326849.1 late promoter-activating protein -
CA268_RS27010 52190..53233 - 1044 WP_029393260.1 DUF968 domain-containing protein -
CA268_RS27015 53261..53440 - 180 WP_000113018.1 hypothetical protein -
CA268_RS27020 53445..53825 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CA268_RS27025 53825..54046 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CA268_RS27030 54229..55785 + 1557 WP_060578235.1 type I restriction-modification system subunit M -
CA268_RS27035 55782..57026 + 1245 WP_086352860.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qacE / sul1 / mph(A) / erm(B) / aac(3)-IIa / blaCTX-M-15 / aadA5 - 1..183508 183508
- inside Integron qacE / aadA5 / dfrA17 - 37..183442 183405


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T76260 WP_001216045.1 NZ_CP021203:c53825-53445 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T76260 NZ_CP021203:c53825-53445 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCAGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT76260 WP_001190712.1 NZ_CP021203:c54046-53825 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT76260 NZ_CP021203:c54046-53825 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References