Detailed information of TA system TA076245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3223514..3224040
• Replicon: chromosome
• Accession: NZ_CP021202
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CA268_RS16630
• Protein ID: WP_000323025.1
• Coordinates: 3223514..3223801 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CA268_RS16635
• Protein ID: WP_000534858.1
• Coordinates: 3223801..3224040 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS16590	3218538..3218753	-	216	WP_000839590.1	phage lysis protein EssD	-
CA268_RS30930	3218973..3219143	+	171	WP_211180497.1	putative zinc-binding protein YnfU	-
CA268_RS16595	3219507..3219722	-	216	WP_000066484.1	cold shock-like protein CspB	-
CA268_RS16600	3220023..3220235	+	213	WP_000087756.1	cold shock-like protein CspF	-
CA268_RS30170	3220290..3220379	+	90	WP_120795389.1	hypothetical protein	-
CA268_RS16605	3220657..3221409	-	753	WP_001047135.1	antitermination protein	-
CA268_RS16610	3221423..3222472	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
CA268_RS16615	3222474..3222752	-	279	WP_012304870.1	hypothetical protein	-
CA268_RS16620	3222819..3223070	-	252	WP_000980994.1	hypothetical protein	-
CA268_RS16625	3223287..3223442	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CA268_RS16630	3223514..3223801	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CA268_RS16635	3223801..3224040	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CA268_RS16640	3224065..3224370	+	306	WP_001326990.1	hypothetical protein	-
CA268_RS16645	3224573..3224905	+	333	WP_001301033.1	protein FlxA	-
CA268_RS16650	3225342..3226655	-	1314	WP_000589005.1	ISNCY family transposase	-
CA268_RS16655	3227139..3228167	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
CA268_RS16660	3228164..3228778	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3187906..3239635	51729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T76245 WP_000323025.1 NZ_CP021202:c3223801-3223514 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T76245 NZ_CP021202:c3223801-3223514 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT76245 WP_000534858.1 NZ_CP021202:c3224040-3223801 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT76245 NZ_CP021202:c3224040-3223801 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8