Detailed information of TA system TA076243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3042241..3042879
• Replicon: chromosome
• Accession: NZ_CP021202
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: CA268_RS15710
• Protein ID: WP_001306887.1
• Coordinates: 3042241..3042417 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CA268_RS15715
• Protein ID: WP_001270286.1
• Coordinates: 3042463..3042879 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS15680	3037297..3037755	+	459	WP_000526135.1	IS200/IS605 family transposase	-
CA268_RS15690	3037863..3039074	-	1212	WP_023143671.1	BenE family transporter YdcO	-
CA268_RS15695	3039127..3039663	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
CA268_RS15700	3039736..3041697	+	1962	WP_057950830.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CA268_RS15705	3041789..3042019	-	231	WP_000494241.1	YncJ family protein	-
CA268_RS15710	3042241..3042417	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CA268_RS15715	3042463..3042879	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CA268_RS15720	3042958..3044364	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
CA268_RS15725	3044609..3045754	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
CA268_RS15730	3045772..3046785	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
CA268_RS15735	3046786..3047727	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3037297..3037755	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T76243 WP_001306887.1 NZ_CP021202:3042241-3042417 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T76243 NZ_CP021202:3042241-3042417 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT76243 WP_001270286.1 NZ_CP021202:3042463-3042879 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT76243 NZ_CP021202:3042463-3042879 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76