Detailed information of TA system TA076231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1471814..1472301
• Replicon: chromosome
• Accession: NZ_CP021202
• Organism: Escherichia coli strain Z1002

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CA268_RS07650
• Protein ID: WP_001520461.1
• Coordinates: 1472050..1472301 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: CA268_RS07645
• Protein ID: WP_001520458.1
• Coordinates: 1471814..1472047 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA268_RS07620	1466887..1467174	+	288	WP_000203747.1	ferredoxin-like protein FixX	-
CA268_RS07625	1467233..1468564	+	1332	WP_001520455.1	MFS transporter	-
CA268_RS07630	1468672..1469202	+	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
CA268_RS07635	1469195..1471057	+	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
CA268_RS07640	1471249..1471728	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
CA268_RS07645	1471814..1472047	+	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CA268_RS07650	1472050..1472301	+	252	WP_001520461.1	CcdB family protein	Toxin
CA268_RS07655	1472361..1473209	-	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
CA268_RS07660	1473216..1473593	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
CA268_RS07665	1473596..1474417	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
CA268_RS07670	1474414..1475403	-	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
CA268_RS07675	1475403..1476689	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T76231 WP_001520461.1 NZ_CP021202:1472050-1472301 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T76231 NZ_CP021202:1472050-1472301 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATTCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT76231 WP_001520458.1 NZ_CP021202:1471814-1472047 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT76231 NZ_CP021202:1471814-1472047 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTATGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4