76215

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 59725..60326 Replicon plasmid pEC-81009
Accession NZ_CP021180
Organism Escherichia coli strain 81009

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag DNNLJILF_RS26320 Protein ID WP_001216034.1
Coordinates 59725..60105 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag DNNLJILF_RS26325 Protein ID WP_001190712.1
Coordinates 60105..60326 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNNLJILF_RS26285 54776..55708 - 933 WP_000081352.1 homocysteine S-methyltransferase -
DNNLJILF_RS26290 55695..57098 - 1404 WP_001373486.1 S-methylmethionine permease -
DNNLJILF_RS26295 57342..58322 + 981 WP_000019407.1 IS5-like element IS5 family transposase -
DNNLJILF_RS26300 58550..58867 + 318 WP_001513659.1 hypothetical protein -
DNNLJILF_RS26305 58817..59230 - 414 Protein_70 DDE-type integrase/transposase/recombinase -
DNNLJILF_RS26310 59154..59513 - 360 WP_001513660.1 hypothetical protein -
DNNLJILF_RS26315 59541..59720 - 180 WP_001513661.1 hypothetical protein -
DNNLJILF_RS26320 59725..60105 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DNNLJILF_RS26325 60105..60326 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DNNLJILF_RS26330 60509..62065 + 1557 WP_001617892.1 type I restriction-modification system subunit M -
DNNLJILF_RS26335 62062..63333 + 1272 WP_023142242.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 senB 1..135720 135720


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T76215 WP_001216034.1 NZ_CP021180:c60105-59725 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T76215 NZ_CP021180:c60105-59725 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT76215 WP_001190712.1 NZ_CP021180:c60326-60105 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT76215 NZ_CP021180:c60326-60105 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References