Detailed information of TA system TA076213

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4816959..4817794
• Replicon: chromosome
• Accession: NZ_CP021179
• Organism: Escherichia coli strain 81009

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: DNNLJILF_RS24760
• Protein ID: WP_000854759.1
• Coordinates: 4816959..4817336 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: DNNLJILF_RS24765
• Protein ID: WP_001295723.1
• Coordinates: 4817426..4817794 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNNLJILF_RS24735	4813070..4814710	-	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
DNNLJILF_RS27230	4815483..4815659	-	177	Protein_4546	helix-turn-helix domain-containing protein	-
DNNLJILF_RS27825	4816023..4816181	-	159	WP_001467148.1	hypothetical protein	-
DNNLJILF_RS24750	4816281..4816457	-	177	WP_000839286.1	DUF957 domain-containing protein	-
DNNLJILF_RS24755	4816474..4816962	-	489	WP_000761690.1	hypothetical protein	-
DNNLJILF_RS24760	4816959..4817336	-	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
DNNLJILF_RS24765	4817426..4817794	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DNNLJILF_RS24775	4817957..4818178	-	222	WP_000692345.1	DUF987 domain-containing protein	-
DNNLJILF_RS24780	4818241..4818717	-	477	WP_001186775.1	RadC family protein	-
DNNLJILF_RS24785	4818733..4819206	-	474	WP_001350782.1	antirestriction protein	-
DNNLJILF_RS24795	4819548..4820366	-	819	WP_001234738.1	DUF945 domain-containing protein	-
DNNLJILF_RS24805	4820521..4820679	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4805313..4831988	26675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T76213 WP_000854759.1 NZ_CP021179:c4817336-4816959 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T76213 NZ_CP021179:c4817336-4816959 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT76213 WP_001295723.1 NZ_CP021179:c4817794-4817426 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT76213 NZ_CP021179:c4817794-4817426 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52