Detailed information of TA system TA076212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4784337..4785157
• Replicon: chromosome
• Accession: NZ_CP021179
• Organism: Escherichia coli strain 81009

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: DNNLJILF_RS24580
• Protein ID: WP_001054379.1
• Coordinates: 4784337..4784594 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: DNNLJILF_RS24585
• Protein ID: WP_000123957.1
• Coordinates: 4784606..4785157 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNNLJILF_RS24550	4779884..4780864	+	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
DNNLJILF_RS24560	4781447..4782553	-	1107	Protein_4515	helicase YjhR	-
DNNLJILF_RS24565	4782616..4783763	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
DNNLJILF_RS24570	4783796..4783960	+	165	Protein_4517	GNAT family N-acetyltransferase	-
DNNLJILF_RS24580	4784337..4784594	+	258	WP_001054379.1	YjhX family toxin	Toxin
DNNLJILF_RS24585	4784606..4785157	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
DNNLJILF_RS24590	4785209..4785955	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
DNNLJILF_RS24595	4786083..4786343	+	261	WP_000077645.1	hypothetical protein	-
DNNLJILF_RS28005	4786381..4786497	+	117	Protein_4522	VOC family protein	-
DNNLJILF_RS24605	4786742..4787863	+	1122	WP_000010833.1	M42 family metallopeptidase	-
DNNLJILF_RS24610	4787860..4788138	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
DNNLJILF_RS24615	4788150..4789463	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE	4772958..4793378	20420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T76212 WP_001054379.1 NZ_CP021179:4784337-4784594 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T76212 NZ_CP021179:4784337-4784594 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT76212 WP_000123957.1 NZ_CP021179:4784606-4785157 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT76212 NZ_CP021179:4784606-4785157 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030