76208

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4173229..4173847 Replicon chromosome
Accession NZ_CP021179
Organism Escherichia coli strain 81009

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DNNLJILF_RS21515 Protein ID WP_001291435.1
Coordinates 4173629..4173847 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DNNLJILF_RS21510 Protein ID WP_000344800.1
Coordinates 4173229..4173603 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNNLJILF_RS21500 4168318..4169511 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DNNLJILF_RS21505 4169534..4172683 + 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
DNNLJILF_RS21510 4173229..4173603 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DNNLJILF_RS21515 4173629..4173847 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DNNLJILF_RS21520 4174021..4174572 + 552 WP_000102539.1 maltose O-acetyltransferase -
DNNLJILF_RS21525 4174688..4175158 + 471 WP_000136192.1 YlaC family protein -
DNNLJILF_RS21530 4175322..4176872 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DNNLJILF_RS21535 4176914..4177267 - 354 WP_000878135.1 DUF1428 family protein -
DNNLJILF_RS21545 4177646..4177957 + 312 WP_000409908.1 MGMT family protein -
DNNLJILF_RS21550 4177988..4178560 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T76208 WP_001291435.1 NZ_CP021179:4173629-4173847 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T76208 NZ_CP021179:4173629-4173847 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT76208 WP_000344800.1 NZ_CP021179:4173229-4173603 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT76208 NZ_CP021179:4173229-4173603 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References