Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2049819..2050333 Replicon chromosome
Accession NZ_CP021178
Organism Staphylococcus aureus subsp. aureus ST398 strain 2012-3

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag CAC37_RS10035 Protein ID WP_000581792.1
Coordinates 2049819..2050028 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag CAC37_RS10040 Protein ID WP_000138932.1
Coordinates 2050040..2050333 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAC37_RS10015 (2045330) 2045330..2046685 - 1356 WP_000611457.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
CAC37_RS10020 (2046700) 2046700..2047770 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
CAC37_RS10025 (2048088) 2048088..2049290 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
CAC37_RS10030 (2049554) 2049554..2049691 - 138 WP_000828354.1 Lmo0850 family protein -
CAC37_RS10035 (2049819) 2049819..2050028 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
CAC37_RS10040 (2050040) 2050040..2050333 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
CAC37_RS10045 (2050499) 2050499..2051983 + 1485 WP_000571550.1 cardiolipin synthase -
CAC37_RS10050 (2052011) 2052011..2052658 + 648 WP_001187614.1 HD domain-containing protein -
CAC37_RS10055 (2053215) 2053215..2053277 + 63 Protein_1940 hypothetical protein -
CAC37_RS10060 (2053255) 2053255..2054570 - 1316 Protein_1941 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T76184 WP_000581792.1 NZ_CP021178:c2050028-2049819 [Staphylococcus aureus subsp. aureus ST398]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T76184 NZ_CP021178:c2050028-2049819 [Staphylococcus aureus subsp. aureus ST398]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT76184 WP_000138932.1 NZ_CP021178:c2050333-2050040 [Staphylococcus aureus subsp. aureus ST398]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT76184 NZ_CP021178:c2050333-2050040 [Staphylococcus aureus subsp. aureus ST398]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References