Detailed information of TA system TA076183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2030082..2030611
• Replicon: chromosome
• Accession: NZ_CP021178
• Organism: Staphylococcus aureus subsp. aureus ST398 strain 2012-3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CAC37_RS09945
• Protein ID: WP_000621175.1
• Coordinates: 2030082..2030444 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CAC37_RS09950
• Protein ID: WP_000948331.1
• Coordinates: 2030441..2030611 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAC37_RS09925 (2027060)	2027060..2027830	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
CAC37_RS09930 (2027805)	2027805..2028284	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
CAC37_RS09935 (2028286)	2028286..2028612	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CAC37_RS09940 (2028731)	2028731..2029732	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CAC37_RS09945 (2030082)	2030082..2030444	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CAC37_RS09950 (2030441)	2030441..2030611	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CAC37_RS09955 (2030696)	2030696..2031844	-	1149	WP_001281139.1	alanine racemase	-
CAC37_RS09960 (2031910)	2031910..2032269	-	360	WP_096002285.1	holo-ACP synthase	-
CAC37_RS09965 (2032273)	2032273..2032764	-	492	WP_001286980.1	PH domain-containing protein	-
CAC37_RS09970 (2032757)	2032757..2034334	-	1578	WP_001294650.1	PH domain-containing protein	-
CAC37_RS09975 (2034327)	2034327..2034806	-	480	WP_001287081.1	hypothetical protein	-
CAC37_RS09980 (2035015)	2035015..2035575	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T76183 WP_000621175.1 NZ_CP021178:c2030444-2030082 [Staphylococcus aureus subsp. aureus ST398]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T76183 NZ_CP021178:c2030444-2030082 [Staphylococcus aureus subsp. aureus ST398]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT76183 WP_000948331.1 NZ_CP021178:c2030611-2030441 [Staphylococcus aureus subsp. aureus ST398]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT76183 NZ_CP021178:c2030611-2030441 [Staphylococcus aureus subsp. aureus ST398]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1