Detailed information of TA system TA076179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1833879..1834655
• Replicon: chromosome
• Accession: NZ_CP021178
• Organism: Staphylococcus aureus subsp. aureus ST398 strain 2012-3

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: CAC37_RS08720
• Protein ID: WP_000031108.1
• Coordinates: 1833879..1834031 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: CAC37_RS08725
• Protein ID: WP_001251224.1
• Coordinates: 1834056..1834655 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAC37_RS08705 (1830084)	1830084..1830905	+	822	WP_000669380.1	RluA family pseudouridine synthase	-
CAC37_RS08710 (1831360)	1831360..1832745	-	1386	WP_000116238.1	class II fumarate hydratase	-
CAC37_RS08715 (1832941)	1832941..1833336	-	396	WP_000901018.1	hypothetical protein	-
CAC37_RS08720 (1833879)	1833879..1834031	-	153	WP_000031108.1	SAS053 family protein	Toxin
CAC37_RS08725 (1834056)	1834056..1834655	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
CAC37_RS08730 (1834814)	1834814..1835284	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
CAC37_RS08735 (1835289)	1835289..1836416	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
CAC37_RS08740 (1836567)	1836567..1837289	-	723	WP_031774878.1	amino acid ABC transporter ATP-binding protein	-
CAC37_RS08745 (1837282)	1837282..1838739	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T76179 WP_000031108.1 NZ_CP021178:c1834031-1833879 [Staphylococcus aureus subsp. aureus ST398]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T76179 NZ_CP021178:c1834031-1833879 [Staphylococcus aureus subsp. aureus ST398]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT76179 WP_001251224.1 NZ_CP021178:c1834655-1834056 [Staphylococcus aureus subsp. aureus ST398]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT76179 NZ_CP021178:c1834655-1834056 [Staphylococcus aureus subsp. aureus ST398]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAACAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4