Detailed information of TA system TA076176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 14721..15313
• Replicon: plasmid p5CRE51-MCR-1
• Accession: NZ_CP021176
• Organism: Escherichia coli strain 5CRE51

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: CA593_RS26300
• Protein ID: WP_000513532.1
• Coordinates: 14721..14969 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: CA593_RS26305
• Protein ID: WP_000681609.1
• Coordinates: 14966..15313 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA593_RS26255	9733..10398	-	666	WP_000532149.1	P-loop NTPase	-
CA593_RS26265	10987..11316	+	330	WP_001542022.1	hypothetical protein	-
CA593_RS26270	11717..12232	+	516	WP_001025393.1	J domain-containing protein	-
CA593_RS26275	12336..12878	+	543	WP_001083904.1	hypothetical protein	-
CA593_RS26280	12875..13129	+	255	WP_001230709.1	hypothetical protein	-
CA593_RS26285	13126..13368	+	243	WP_000151456.1	hypothetical protein	-
CA593_RS26295	13939..14232	+	294	WP_000673428.1	hypothetical protein	-
CA593_RS26300	14721..14969	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
CA593_RS26305	14966..15313	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CA593_RS26310	15401..15583	-	183	WP_001428470.1	hypothetical protein	-
CA593_RS26315	16085..16384	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
CA593_RS26320	16644..16970	-	327	WP_000206697.1	hypothetical protein	-
CA593_RS26325	16972..17412	-	441	WP_023223352.1	hypothetical protein	-
CA593_RS26330	17432..18139	-	708	WP_000108794.1	hypothetical protein	-
CA593_RS26335	18141..18893	-	753	WP_000797966.1	conjugal transfer protein TraL	-
CA593_RS26340	18911..19312	-	402	WP_021575837.1	hypothetical protein	-
CA593_RS26345	19680..20021	+	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..60961	60961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T76176 WP_000513532.1 NZ_CP021176:14721-14969 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T76176 NZ_CP021176:14721-14969 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT76176 WP_000681609.1 NZ_CP021176:14966-15313 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT76176 NZ_CP021176:14966-15313 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49