Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 12875..13368 Replicon plasmid p5CRE51-MCR-1
Accession NZ_CP021176
Organism Escherichia coli strain 5CRE51

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag CA593_RS26280 Protein ID WP_001230709.1
Coordinates 12875..13129 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag CA593_RS26285 Protein ID WP_000151456.1
Coordinates 13126..13368 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA593_RS26240 8450..8611 + 162 WP_001366886.1 type I toxin-antitoxin system Hok family toxin -
CA593_RS26245 8761..9357 + 597 WP_000125165.1 hypothetical protein -
CA593_RS28175 9375..9659 - 285 WP_001162371.1 hypothetical protein -
CA593_RS26255 9733..10398 - 666 WP_000532149.1 P-loop NTPase -
CA593_RS26265 10987..11316 + 330 WP_001542022.1 hypothetical protein -
CA593_RS26270 11717..12232 + 516 WP_001025393.1 J domain-containing protein -
CA593_RS26275 12336..12878 + 543 WP_001083904.1 hypothetical protein -
CA593_RS26280 12875..13129 + 255 WP_001230709.1 hypothetical protein Toxin
CA593_RS26285 13126..13368 + 243 WP_000151456.1 hypothetical protein Antitoxin
CA593_RS26295 13939..14232 + 294 WP_000673428.1 hypothetical protein -
CA593_RS26300 14721..14969 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
CA593_RS26305 14966..15313 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
CA593_RS26310 15401..15583 - 183 WP_001428470.1 hypothetical protein -
CA593_RS26315 16085..16384 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
CA593_RS26320 16644..16970 - 327 WP_000206697.1 hypothetical protein -
CA593_RS26325 16972..17412 - 441 WP_023223352.1 hypothetical protein -
CA593_RS26330 17432..18139 - 708 WP_000108794.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..60961 60961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type I toxin-antitoxin system, toxin Ldr

(1-35)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T76175 WP_001230709.1 NZ_CP021176:12875-13129 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T76175 NZ_CP021176:12875-13129 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT76175 WP_000151456.1 NZ_CP021176:13126-13368 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT76175 NZ_CP021176:13126-13368 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References