Detailed information of TA system TA076163

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3406095..3406930
• Replicon: chromosome
• Accession: NZ_CP021175
• Organism: Escherichia coli strain 5CRE51

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: CA593_RS17455
• Protein ID: WP_000854761.1
• Coordinates: 3406095..3406472 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: CA593_RS17460
• Protein ID: WP_001295723.1
• Coordinates: 3406562..3406930 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA593_RS17425	3401484..3401666	-	183	WP_024167529.1	ethanolamine utilization protein	-
CA593_RS17430	3402120..3402509	-	390	WP_001445118.1	IS110 family transposase	-
CA593_RS17435	3403003..3403407	+	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
CA593_RS17440	3403404..3403751	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
CA593_RS17445	3403800..3405335	+	1536	WP_001333339.1	IS66-like element ISEc22 family transposase	-
CA593_RS27785	3405770..3405883	-	114	WP_001161660.1	DUF957 domain-containing protein	-
CA593_RS17450	3405896..3406098	-	203	Protein_3194	hypothetical protein	-
CA593_RS17455	3406095..3406472	-	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
CA593_RS17460	3406562..3406930	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CA593_RS17470	3407093..3407314	-	222	WP_000692345.1	DUF987 domain-containing protein	-
CA593_RS17475	3407377..3407853	-	477	WP_001186711.1	RadC family protein	-
CA593_RS17480	3407868..3408341	-	474	WP_000855059.1	antirestriction protein	-
CA593_RS17490	3408683..3409501	-	819	WP_001234729.1	DUF945 domain-containing protein	-
CA593_RS17500	3409656..3409814	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T76163 WP_000854761.1 NZ_CP021175:c3406472-3406095 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T76163 NZ_CP021175:c3406472-3406095 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT76163 WP_001295723.1 NZ_CP021175:c3406930-3406562 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT76163 NZ_CP021175:c3406930-3406562 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52