Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 401616..403041 Replicon chromosome
Accession NZ_CP021175
Organism Escherichia coli strain 5CRE51

Toxin (Protein)


Gene name rnlA Uniprot ID A0A1X9TDS5
Locus tag CA593_RS02090 Protein ID WP_001730411.1
Coordinates 401971..403041 (-) Length 357 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID E6BSF0
Locus tag CA593_RS02085 Protein ID WP_000461705.1
Coordinates 401616..401978 (-) Length 121 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA593_RS02070 397633..398841 - 1209 WP_000772656.1 tyrosine-type recombinase/integrase -
CA593_RS02075 399444..400583 + 1140 WP_057077950.1 hypothetical protein -
CA593_RS02080 401209..401478 + 270 WP_000429347.1 hypothetical protein -
CA593_RS02085 401616..401978 - 363 WP_000461705.1 type II toxin-antitoxin system RnlB family antitoxin Antitoxin
CA593_RS02090 401971..403041 - 1071 WP_001730411.1 type II toxin-antitoxin system RnlA family toxin Toxin
CA593_RS02095 403176..403463 - 288 Protein_372 recombinase family protein -
CA593_RS02100 403493..403999 + 507 WP_108084473.1 hypothetical protein -
CA593_RS02105 404002..404412 + 411 WP_001032316.1 tail fiber assembly protein -
CA593_RS02110 404393..404626 - 234 WP_001619155.1 hypothetical protein -
CA593_RS02115 405487..407622 - 2136 Protein_376 prophage tail fiber N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 397633..459442 61809


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(15-112)


Sequences

Toxin        


Download         Length: 357 a.a.        Molecular weight: 40463.36 Da        Isoelectric Point: 6.8748

>T76151 WP_001730411.1 NZ_CP021175:c403041-401971 [Escherichia coli]
MTVRNYTNLNLDRTTIEATSRAFIENKNYSVHSIGPMPGARAGLRVVFAKPGEALATVNIFYNNGGTSTVQYQTGANHNL
GKELADDLYETINPAEFEQVNMVLQGFVEANVLSVLQLSAEQPHIQFYEYLRNTHTTVWKIHSPEFQDELTVSLHHRNGT
LQIQGRPLSCYRVFIFNLSELLDLQGLEKVLIRQDDGKAYIVQKEVARSHLECEMGDAYPLLHKNVEKLLVSGLCVKLAA
PDLPDYCMLLYPELRSVEGVLKSTMYRYGMPITQDGFGRYFDKIGSEFILKQQFGCNLSSAKVKTINDAYTFFNRERHGL
FHMEIVVDTSRMVSDMARLMTKARQAWGIIKDLYIV

Download         Length: 1071 bp

>T76151 NZ_CP021175:c403041-401971 [Escherichia coli]
ATGACAGTCAGGAACTACACTAATTTAAATTTAGATCGGACAACTATTGAGGCTACGTCTAGGGCATTTATTGAAAATAA
AAACTATTCCGTCCATTCAATTGGACCAATGCCTGGTGCAAGAGCAGGACTTCGGGTTGTGTTTGCTAAACCAGGAGAAG
CTTTGGCAACTGTTAACATTTTCTATAATAACGGAGGGACGTCTACAGTCCAGTATCAAACAGGAGCTAATCATAATCTT
GGAAAAGAGTTGGCTGATGATCTCTATGAAACCATCAATCCTGCAGAATTTGAACAGGTAAATATGGTGTTACAAGGGTT
CGTTGAAGCGAACGTTTTATCTGTACTTCAGCTCTCAGCAGAGCAGCCACATATACAATTTTACGAATACTTACGGAACA
CTCATACAACGGTGTGGAAAATTCATTCGCCAGAATTTCAGGATGAGTTGACAGTTAGTCTGCATCACAGAAATGGAACA
CTACAAATACAAGGGCGTCCATTGTCATGTTATAGAGTGTTTATATTCAACTTGTCAGAGCTACTCGATTTACAAGGACT
GGAAAAAGTTCTCATTCGTCAGGACGATGGCAAAGCGTATATAGTTCAGAAAGAAGTTGCTCGCTCACATCTAGAGTGTG
AAATGGGGGATGCGTACCCACTATTGCATAAGAATGTTGAAAAATTGCTCGTTTCTGGGCTGTGTGTTAAACTCGCCGCC
CCCGATTTACCTGATTACTGTATGTTGTTGTATCCTGAATTACGTTCAGTTGAGGGAGTACTTAAATCAACAATGTACCG
ATATGGCATGCCAATCACGCAGGATGGTTTCGGGCGTTATTTTGATAAAATTGGGAGTGAATTTATTCTGAAACAACAAT
TTGGATGCAATTTATCGTCAGCTAAGGTTAAGACCATTAATGATGCCTATACTTTCTTTAACAGAGAACGTCATGGCCTG
TTTCATATGGAGATAGTTGTCGACACAAGCCGTATGGTTTCTGACATGGCACGACTGATGACTAAGGCCAGACAGGCATG
GGGAATTATTAAGGATTTGTATATTGTTTGA

Antitoxin


Download         Length: 121 a.a.        Molecular weight: 13304.23 Da        Isoelectric Point: 4.2386

>AT76151 WP_000461705.1 NZ_CP021175:c401978-401616 [Escherichia coli]
MFEITGVNVSGALIAVVMATGYENPLTSVHEIEKRLSVILGNSQATGEVLFDLLCSNGPEWNRFVSLEIRCGSILLDTAK
IVDEQEIPAQILRHQRDVLRNHPEYVEDSVLTPDDVLCIL

Download         Length: 363 bp

>AT76151 NZ_CP021175:c401978-401616 [Escherichia coli]
TTGTTTGAAATAACAGGTGTTAACGTCTCAGGGGCTCTCATTGCCGTCGTTATGGCGACTGGCTACGAGAACCCCTTGAC
TTCAGTTCACGAAATCGAGAAGAGGCTTTCTGTTATACTGGGCAATTCACAGGCTACAGGTGAGGTTTTGTTTGACCTTT
TGTGTTCTAATGGTCCAGAGTGGAATCGCTTTGTTTCGCTGGAAATAAGGTGTGGCAGTATTTTGCTGGATACTGCCAAA
ATTGTGGATGAGCAAGAAATTCCAGCGCAGATCTTGAGACATCAGAGAGATGTATTACGTAACCACCCTGAGTACGTAGA
AGACTCTGTGTTAACACCAGATGATGTTCTTTGTATTCTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X9TDS5

Antitoxin

Source ID Structure
AlphaFold DB A0A1X9TDS3

References