76125

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1270780..1271696 Replicon chromosome
Accession NZ_CP021169
Organism Bacillus subtilis strain TLO3

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag B9N48_RS06755 Protein ID WP_003244695.1
Coordinates 1270950..1271696 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag B9N48_RS06750 Protein ID WP_003232646.1
Coordinates 1270780..1270950 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9N48_RS06705 1266643..1267221 + 579 WP_014476551.1 YmfQ family protein -
B9N48_RS06710 1267218..1267490 + 273 WP_015715738.1 hypothetical protein -
B9N48_RS06715 1267493..1267705 + 213 Protein_1247 terminase -
B9N48_RS06720 1267731..1267973 + 243 Protein_1248 phage portal protein -
B9N48_RS06725 1267970..1268134 + 165 WP_041850927.1 XkdX family protein -
B9N48_RS06730 1268178..1269017 + 840 WP_041850926.1 phage-like element PBSX protein XepA -
B9N48_RS06735 1269070..1269339 + 270 WP_003232655.1 hemolysin XhlA family protein -
B9N48_RS06740 1269352..1269615 + 264 WP_003232653.1 phage holin -
B9N48_RS06745 1269628..1270521 + 894 WP_041850925.1 N-acetylmuramoyl-L-alanine amidase -
B9N48_RS20560 1270558..1270695 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
B9N48_RS06750 1270780..1270950 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
B9N48_RS06755 1270950..1271696 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
B9N48_RS06760 1271806..1272807 - 1002 WP_003232642.1 inorganic phosphate transporter -
B9N48_RS06765 1272820..1273437 - 618 WP_003218470.1 DUF47 domain-containing protein -
B9N48_RS06770 1273713..1275029 - 1317 WP_041850924.1 serine/threonine exchanger -
B9N48_RS06775 1275417..1276367 + 951 WP_041344800.1 ring-cleaving dioxygenase -
B9N48_RS20565 1276468..1276614 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T76125 WP_003244695.1 NZ_CP021169:c1271696-1270950 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T76125 NZ_CP021169:c1271696-1270950 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGTGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT76125 WP_003232646.1 NZ_CP021169:c1270950-1270780 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT76125 NZ_CP021169:c1270950-1270780 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References