Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 508597..509233 Replicon chromosome
Accession NZ_CP021169
Organism Bacillus subtilis strain TLO3

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag B9N48_RS02605 Protein ID WP_003156187.1
Coordinates 508883..509233 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag B9N48_RS02600 Protein ID WP_003225183.1
Coordinates 508597..508878 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9N48_RS02580 504956..505555 - 600 WP_042977090.1 rhomboid family intramembrane serine protease -
B9N48_RS02585 505650..506015 + 366 WP_014475874.1 holo-ACP synthase -
B9N48_RS02590 506181..507197 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
B9N48_RS02595 507312..508481 + 1170 WP_017697002.1 alanine racemase -
B9N48_RS02600 508597..508878 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
B9N48_RS02605 508883..509233 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B9N48_RS02610 509348..510172 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
B9N48_RS02615 510177..510542 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
B9N48_RS02620 510546..510947 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
B9N48_RS02625 510959..511966 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
B9N48_RS02630 512028..512357 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
B9N48_RS02635 512354..512836 + 483 WP_021481702.1 anti-sigma B factor RsbW -
B9N48_RS02640 512802..513590 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
B9N48_RS02645 513590..514189 + 600 WP_003234303.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T76124 WP_003156187.1 NZ_CP021169:508883-509233 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T76124 NZ_CP021169:508883-509233 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT76124 WP_003225183.1 NZ_CP021169:508597-508878 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT76124 NZ_CP021169:508597-508878 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References