Detailed information of TA system TA076109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 78620..79263
• Replicon: plasmid p203
• Accession: NZ_CP021166
• Organism: Klebsiella pneumoniae strain 203

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: BH367_RS26525
• Protein ID: WP_016236302.1
• Coordinates: 78620..79036 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: BH367_RS26530
• Protein ID: WP_001261282.1
• Coordinates: 79033..79263 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH367_RS26505	73988..74338	+	351	WP_004187110.1	DUF305 domain-containing protein	-
BH367_RS26510	74524..75438	-	915	WP_153378766.1	HNH endonuclease	-
BH367_RS26515	75977..76999	-	1023	WP_032439672.1	helicase UvrD	-
BH367_RS26520	76984..78546	-	1563	WP_004206609.1	AAA family ATPase	-
BH367_RS26525	78620..79036	-	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
BH367_RS26530	79033..79263	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BH367_RS26535	79220..79642	+	423	WP_046624301.1	hypothetical protein	-
BH367_RS26545	80183..80614	+	432	WP_174805835.1	hypothetical protein	-
BH367_RS26550	81232..82686	-	1455	WP_072001611.1	EAL domain-containing protein	-
BH367_RS26555	83290..83547	+	258	WP_023292103.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15 / dfrA14 / aac(6')-Ib-cr / blaOXA-1 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A)	-	1..177145	177145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T76109 WP_016236302.1 NZ_CP021166:c79036-78620 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T76109 NZ_CP021166:c79036-78620 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT76109 WP_001261282.1 NZ_CP021166:c79263-79033 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT76109 NZ_CP021166:c79263-79033 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3