Detailed information of TA system TA076060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 12628..13265
• Replicon: plasmid unnamed2
• Accession: NZ_CP021160
• Organism: Burkholderia glumae strain 411gr-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C5AP38
• Locus tag: B7760_RS29390
• Protein ID: WP_012735347.1
• Coordinates: 12628..13029 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: B7760_RS29395
• Protein ID: WP_017924531.1
• Coordinates: 13029..13265 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7760_RS29355 (B7760_05897)	7842..8276	-	435	WP_161522145.1	helix-turn-helix domain-containing protein	-
B7760_RS29360 (B7760_05898)	8386..9002	+	617	Protein_11	NAD(P)H-dependent oxidoreductase	-
B7760_RS29365 (B7760_05899)	9007..9660	+	654	WP_161522129.1	SDR family oxidoreductase	-
B7760_RS29370 (B7760_05900)	9690..10601	+	912	WP_161522130.1	oxidoreductase	-
B7760_RS29375	10625..11265	+	641	Protein_14	DsbA family protein	-
B7760_RS29380 (B7760_05902)	11350..11472	+	123	Protein_15	AAA family ATPase	-
B7760_RS29385 (B7760_05903)	11671..12399	-	729	WP_012735342.1	IS6 family transposase	-
B7760_RS29390 (B7760_05904)	12628..13029	-	402	WP_012735347.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7760_RS29395 (B7760_05905)	13029..13265	-	237	WP_017924531.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B7760_RS29400 (B7760_05906)	13402..14061	-	660	WP_161522132.1	tyrosine-type recombinase/integrase	-
B7760_RS29405 (B7760_05907)	14176..14466	-	291	WP_161522133.1	hypothetical protein	-
B7760_RS29410 (B7760_05908)	14506..14673	-	168	WP_174525173.1	hypothetical protein	-
B7760_RS29415 (B7760_05909)	14843..15013	-	171	WP_174525174.1	hypothetical protein	-
B7760_RS29420 (B7760_05910)	15084..15812	-	729	WP_012735342.1	IS6 family transposase	-
B7760_RS29425	15997..16218	+	222	WP_017433768.1	hypothetical protein	-
B7760_RS29430 (B7760_05911)	16440..16904	+	465	WP_012733846.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..169072	169072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14847.12 Da
• Isoelectric Point: 5.2138

>T76060 WP_012735347.1 NZ_CP021160:c13029-12628 [Burkholderia glumae]
MPRFMLDTNMCIYLMKNQPEQVAKRFAECFVGDVVMSAITYAELEYGVAVSDNRAKERRNLAALIEDILVAPFDAAAAVA
YGPIREATRERKKDHLDKLIAAHAVALDVVLVTNNAKDFASYPGIKLDNWLND

Nucleotide

• Length: 402 bp

>T76060 NZ_CP021160:c13029-12628 [Burkholderia glumae]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8965.10 Da
• Isoelectric Point: 6.3676

>AT76060 WP_017924531.1 NZ_CP021160:c13265-13029 [Burkholderia glumae]
MQTTRVFRNGNSQAVRIPADLAYERSDVELEIERVGDEIRIRPARRPLTGVLKKFAKFSQDFMAEGRGEQEQAEREGL

Nucleotide

• Length: 237 bp

>AT76060 NZ_CP021160:c13265-13029 [Burkholderia glumae]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTCTGTAA

Structures

Toxin

• AlphaFold DB: C5AP38