Detailed information of TA system TA076059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 58523..59060
• Replicon: plasmid unnamed1
• Accession: NZ_CP021159
• Organism: Burkholderia glumae strain 411gr-6

Toxin (Protein)

• Gene name: parE
• Uniprot ID: C5AN85
• Locus tag: B7760_RS28600
• Protein ID: WP_012734112.1
• Coordinates: 58523..58825 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: C5AN86
• Locus tag: B7760_RS28605
• Protein ID: WP_012734113.1
• Coordinates: 58815..59060 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7760_RS28555 (B7760_05718)	54910..55251	-	342	WP_161522030.1	hypothetical protein	-
B7760_RS28560 (B7760_05719)	55248..55583	-	336	WP_174525154.1	hypothetical protein	-
B7760_RS28565 (B7760_05720)	55580..55819	-	240	WP_161522032.1	hypothetical protein	-
B7760_RS28570 (B7760_05721)	55832..56005	-	174	WP_161522033.1	hypothetical protein	-
B7760_RS28575 (B7760_05723)	56197..56412	-	216	WP_161522034.1	hypothetical protein	-
B7760_RS28580 (B7760_05724)	56674..56991	-	318	WP_148271069.1	hypothetical protein	-
B7760_RS31065 (B7760_05725)	57175..57357	-	183	WP_252979824.1	hypothetical protein	-
B7760_RS28590 (B7760_05726)	57459..57854	-	396	WP_252979825.1	hypothetical protein	-
B7760_RS28595 (B7760_05727)	58121..58363	-	243	WP_043308436.1	hypothetical protein	-
B7760_RS28600 (B7760_05728)	58523..58825	-	303	WP_012734112.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7760_RS28605 (B7760_05729)	58815..59060	-	246	WP_012734113.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
B7760_RS28610 (B7760_05730)	59212..59406	-	195	WP_043308437.1	hypothetical protein	-
B7760_RS28615 (B7760_05731)	60722..61393	+	672	WP_012734035.1	AAA family ATPase	-
B7760_RS28620 (B7760_05732)	61393..62358	+	966	WP_012734036.1	ParB/RepB/Spo0J family partition protein	-
B7760_RS28625 (B7760_05733)	63113..63970	+	858	WP_012734037.1	plasmid replication initiator TrfA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..193251	193251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-92)

Antitoxin

• Antitoxin ParD (1-80)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11548.11 Da
• Isoelectric Point: 6.7171

>T76059 WP_012734112.1 NZ_CP021159:c58825-58523 [Burkholderia glumae]
MRGKARVVRLTPLAEADLEDIWAYIFEQWSLAQAEQYVGEIVAAFNRLARGEWVGAVRGEPYRRYLVGFHAVFYRETTDA
LDVACVRHQRMDVGSHLSQY

Nucleotide

• Length: 303 bp

>T76059 NZ_CP021159:c58825-58523 [Burkholderia glumae]
ATGCGGGGTAAGGCGCGGGTGGTGCGGCTGACGCCCTTGGCCGAGGCCGACCTCGAGGACATCTGGGCTTACATCTTCGA
GCAGTGGTCACTCGCGCAGGCCGAGCAATACGTCGGCGAGATCGTCGCTGCCTTCAACCGACTGGCGCGCGGCGAATGGG
TCGGGGCCGTGCGGGGCGAACCTTACCGGCGCTATCTGGTCGGCTTCCATGCGGTGTTTTATCGAGAGACCACCGACGCC
TTGGATGTCGCCTGTGTGCGGCATCAGCGTATGGACGTGGGTTCGCACCTGTCGCAGTATTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8645.69 Da
• Isoelectric Point: 7.2029

>AT76059 WP_012734113.1 NZ_CP021159:c59060-58815 [Burkholderia glumae]
MPRNTSISLGDHLTQFVDTQVASGRYGSASDVVRAGLRLLETQETELKALKEALRASEASGAPAAFDGQAFLDRMRAKHA
G

Nucleotide

• Length: 246 bp

>AT76059 NZ_CP021159:c59060-58815 [Burkholderia glumae]
ATGCCGCGGAACACATCGATCTCGCTGGGGGATCACCTGACTCAGTTTGTCGACACACAGGTCGCATCCGGCCGCTATGG
CTCGGCCAGCGACGTGGTGCGCGCCGGGCTTCGCCTGCTCGAGACGCAGGAAACCGAACTGAAGGCGCTGAAGGAGGCGT
TGCGCGCCAGTGAGGCGTCCGGCGCGCCCGCGGCGTTTGACGGCCAAGCGTTCCTCGACCGCATGCGTGCGAAGCATGCG
GGGTAA

Structures

Toxin

• AlphaFold DB: C5AN85

Antitoxin

• AlphaFold DB: C5AN86