Detailed information of TA system TA076055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 7996..9343
• Replicon: plasmid pLA16-4
• Accession: NZ_CP021150
• Organism: Vibrio campbellii strain LA16-V1

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: CAY59_RS28395
• Protein ID: WP_086370885.1
• Coordinates: 8402..9343 (+)
• Length: 314 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: CAY59_RS28390
• Protein ID: WP_085343578.1
• Coordinates: 7996..8400 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY59_RS28370	5672..6124	-	453	WP_021018079.1	hypothetical protein	-
CAY59_RS28380	6692..6934	+	243	WP_086369808.1	hypothetical protein	-
CAY59_RS28385	7214..7807	+	594	WP_085343577.1	tyrosine-type recombinase/integrase	-
CAY59_RS28390	7996..8400	+	405	WP_085343578.1	S-adenosylhomocysteine hydrolase	Antitoxin
CAY59_RS28395	8402..9343	+	942	WP_086370885.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CAY59_RS28400	9363..10880	+	1518	WP_086370886.1	type I restriction-modification system subunit M	-
CAY59_RS28405	10870..12129	+	1260	WP_086370887.1	restriction endonuclease subunit S	-
CAY59_RS28410	12195..13031	+	837	WP_086370888.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63992	63992

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (37-300)

Antitoxin

• Protein of unknown function DUF6088 (7-130)

Sequences

Toxin

Protein

• Length: 314 a.a.
• Molecular weight: 35941.37 Da
• Isoelectric Point: 5.4876

>T76055 WP_086370885.1 NZ_CP021150:8402-9343 [Vibrio campbellii]
MNTLKQQFLEVSDALELGNPAIIEKDYWVVALLAELAKVCPEHHQMVFSGGTALAKSNVKILRMSEDVDIKLIPNQAFDE
LSRARKKAARKACINEIEQAITQSGRFNVETRSVRDEYRYVEFELKYPQQFSQAPCLRPIIKLELIETIPLLDVESRSIQ
SLVAELYRQPHEVSAFDCVSVYTTLIEKVISMLRRTMSVKRNTERSDDVALVRHIYDVHCITKLSMPELDSIDGLFKAVL
EEDIARFGNQHAEFVANPKQELQLGLKEIEENAIFRQRFQEFVTPMVFNTEPHDFDTCFASFKRIAASLIEKI

Nucleotide

• Length: 942 bp

>T76055 NZ_CP021150:8402-9343 [Vibrio campbellii]
ATGAACACACTAAAACAACAGTTCCTTGAGGTTTCCGATGCGCTTGAGCTCGGAAACCCAGCCATCATTGAAAAAGATTA
CTGGGTCGTGGCTTTACTGGCTGAGCTAGCGAAAGTTTGTCCTGAGCATCATCAAATGGTGTTTTCTGGCGGCACTGCGT
TGGCGAAATCAAACGTTAAAATTCTAAGAATGTCGGAAGATGTGGATATAAAACTCATCCCCAACCAAGCATTTGATGAA
CTGAGCCGAGCGAGAAAGAAAGCTGCGAGAAAGGCATGTATAAATGAGATTGAACAGGCGATCACCCAGTCAGGGCGATT
TAACGTTGAAACGCGCTCTGTGCGAGATGAGTACCGCTACGTCGAGTTTGAACTTAAATACCCGCAGCAGTTTAGTCAAG
CACCCTGTCTTCGCCCCATCATCAAGTTAGAGTTAATCGAAACCATTCCATTACTGGATGTAGAGTCGCGCAGCATTCAA
TCTCTGGTTGCAGAGTTGTACCGACAACCGCACGAAGTGAGTGCGTTTGATTGCGTTTCTGTCTATACCACACTAATTGA
AAAAGTTATTTCGATGCTTCGCCGTACCATGTCGGTAAAACGCAATACGGAGCGAAGCGATGATGTTGCTTTAGTTCGCC
ACATCTACGATGTGCACTGCATAACAAAGCTCTCAATGCCAGAACTCGACTCGATTGACGGCCTATTCAAAGCGGTTTTG
GAAGAAGATATTGCCCGCTTTGGCAATCAACATGCAGAGTTTGTTGCCAACCCCAAACAAGAGCTACAACTTGGCCTCAA
AGAGATTGAGGAAAATGCAATATTCCGCCAACGCTTTCAGGAATTTGTCACGCCAATGGTGTTTAACACCGAACCCCATG
ATTTTGATACCTGCTTTGCCTCATTTAAACGTATTGCAGCATCGCTAATTGAAAAAATTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15186.50 Da
• Isoelectric Point: 10.5319

>AT76055 WP_085343578.1 NZ_CP021150:7996-8400 [Vibrio campbellii]
MTAADRIYQKIKRSRRYVFERKDFDGFASYDQIGRALRQLVQQGELMKLGYGLYTKARINSLTGKPMPTNPGGTDALMRE
ILKMKGVDFEMDSLSLQSLSGSSTQIPSSIQYSWDPKQFNRKLVVGSRVLNLPS

Nucleotide

• Length: 405 bp

>AT76055 NZ_CP021150:7996-8400 [Vibrio campbellii]
ATGACAGCAGCAGACCGCATTTATCAAAAGATAAAGCGTTCAAGACGTTATGTATTTGAACGCAAAGACTTTGATGGATT
TGCAAGTTACGACCAAATAGGTCGGGCCCTGAGGCAGCTTGTGCAGCAAGGTGAGTTAATGAAGCTTGGCTATGGCTTGT
ATACCAAAGCAAGAATCAATAGCCTCACTGGCAAGCCCATGCCGACTAATCCGGGAGGGACCGATGCCCTTATGCGAGAA
ATTCTTAAAATGAAAGGCGTTGATTTTGAGATGGATAGTCTATCGCTACAAAGTCTTTCAGGTTCAAGCACCCAAATTCC
ATCTTCTATTCAGTATTCATGGGACCCAAAACAGTTTAATCGCAAACTGGTGGTTGGTAGCCGTGTGCTGAACTTACCAA
GTTAG