Detailed information of TA system TA076054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 48922..49490
• Replicon: plasmid pLA16-3
• Accession: NZ_CP021149
• Organism: Vibrio campbellii strain LA16-V1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CAY59_RS28280
• Protein ID: WP_086370874.1
• Coordinates: 49149..49490 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CAY59_RS28275
• Protein ID: WP_017449238.1
• Coordinates: 48922..49152 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY59_RS28240	44316..44726	-	411	WP_086370871.1	Ig-like domain-containing protein	-
CAY59_RS28245	44747..45310	-	564	WP_172841007.1	Ig-like domain-containing protein	-
CAY59_RS28250	45836..46234	-	399	WP_157664732.1	hypothetical protein	-
CAY59_RS28255	46544..46765	+	222	WP_023624649.1	hypothetical protein	-
CAY59_RS28265	47368..47751	+	384	Protein_46	recombinase family protein	-
CAY59_RS28270	47746..48471	-	726	WP_017449237.1	site-specific integrase	-
CAY59_RS28275	48922..49152	+	231	WP_017449238.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CAY59_RS28280	49149..49490	+	342	WP_086370874.1	endoribonuclease MazF	Toxin
CAY59_RS28285	49793..50368	-	576	WP_086370875.1	hypothetical protein	-
CAY59_RS28290	50574..51542	+	969	WP_086369237.1	IS30-like element ISVa6 family transposase	-
CAY59_RS28295	51474..52193	-	720	WP_086370876.1	AHH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67308	67308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-108)

Antitoxin

• Antitoxin PemI-like (1-75)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12185.73 Da
• Isoelectric Point: 5.8124

>T76054 WP_086370874.1 NZ_CP021149:49149-49490 [Vibrio campbellii]
MTYAVPEKGDVVVMIFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Nucleotide

• Length: 342 bp

>T76054 NZ_CP021149:49149-49490 [Vibrio campbellii]
ATGACGTATGCAGTACCAGAGAAAGGCGATGTAGTAGTAATGATTTTTAATCCGCAAAGCGGACACGAACAAGCGGGAGA
CAGTCGGCCTGGGCTGGTCATTTCCCCGAAAGAGTTTAACCAAGCAACTGGCTTTGCTCTGGTATGCCCCATAACCAACC
AGTCAAAAGGGTATCCGTTTGAAGTTGAAATAACCGGAAGCCAAAAAACAACGGGTGTGGTGCTGGCTGACCAAGTGAAG
TCCTTGGATTGGCGCGCGCGTCGCGCTCGTGTGGTGGACTCCGTTTCAAACGAAACACTGGGGACTGTGCGGGATTATGT
CACAGCGATTGCTAATGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8499.87 Da
• Isoelectric Point: 6.4604

>AT76054 WP_017449238.1 NZ_CP021149:48922-49152 [Vibrio campbellii]
MSQTIAKWGHSAAVRIPSKVLKNMDMAVGDKVEVKVFNRQIIIEPAKPTLAQLLSQITDENRHDEVITETMGNELL

Nucleotide

• Length: 231 bp

>AT76054 NZ_CP021149:48922-49152 [Vibrio campbellii]
ATGTCACAAACAATAGCTAAGTGGGGCCACAGCGCAGCTGTGCGTATACCTTCTAAGGTATTGAAAAATATGGATATGGC
GGTGGGTGACAAAGTGGAGGTTAAAGTGTTCAATCGACAAATTATCATTGAACCTGCAAAACCAACACTCGCCCAGCTTT
TGAGTCAGATCACTGATGAAAACCGCCATGATGAAGTAATCACCGAAACTATGGGTAATGAATTGTTATGA