Detailed information of TA system TA076050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4427431..4428268
• Replicon: chromosome
• Accession: NZ_CP021144
• Organism: Shigella sonnei strain 2015AM-1099

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: B9127_RS24410
• Protein ID: WP_000227784.1
• Coordinates: 4427431..4427973 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: B9127_RS24415
• Protein ID: WP_001297137.1
• Coordinates: 4427957..4428268 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9127_RS24370 (4422496)	4422496..4422987	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
B9127_RS24375 (4423115)	4423115..4424479	-	1365	WP_001000971.1	MFS transporter	-
B9127_RS24385 (4424598)	4424598..4425295	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
B9127_RS24390 (4425664)	4425664..4426061	+	398	Protein_4371	sel1 repeat family protein	-
B9127_RS24405 (4426809)	4426809..4427375	+	567	Protein_4373	SEL1-like repeat protein	-
B9127_RS24410 (4427431)	4427431..4427973	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
B9127_RS24415 (4427957)	4427957..4428268	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
B9127_RS24420 (4428453)	4428453..4429343	-	891	WP_000971327.1	heme o synthase	-
B9127_RS24425 (4429355)	4429355..4429684	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
B9127_RS24430 (4429684)	4429684..4430298	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
B9127_RS24435 (4430288)	4430288..4432279	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
B9127_RS24440 (4432301)	4432301..4433248	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T76050 WP_000227784.1 NZ_CP021144:c4427973-4427431 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T76050 NZ_CP021144:c4427973-4427431 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT76050 WP_001297137.1 NZ_CP021144:c4428268-4427957 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT76050 NZ_CP021144:c4428268-4427957 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3