Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3695115..3695612 Replicon chromosome
Accession NZ_CP021144
Organism Shigella sonnei strain 2015AM-1099

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag B9127_RS20520 Protein ID WP_001173343.1
Coordinates 3695439..3695612 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag B9127_RS20515 Protein ID WP_000398619.1
Coordinates 3695115..3695411 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9127_RS20490 (3691846) 3691846..3692565 - 720 WP_000611285.1 two-component system response regulator DcuR -
B9127_RS20495 (3692562) 3692562..3694193 - 1632 WP_001216495.1 sensor histidine kinase -
B9127_RS20500 (3694374) 3694374..3694604 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
B9127_RS20505 (3694616) 3694616..3694888 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
B9127_RS20515 (3695115) 3695115..3695411 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
B9127_RS20520 (3695439) 3695439..3695612 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
B9127_RS20525 (3695731) 3695731..3697248 - 1518 WP_001295086.1 lysine--tRNA ligase -
B9127_RS20530 (3697485) 3697485..3698942 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
B9127_RS20535 (3699001) 3699001..3699258 - 258 Protein_3667 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T76048 WP_001173343.1 NZ_CP021144:3695439-3695612 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T76048 NZ_CP021144:3695439-3695612 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT76048 WP_000398619.1 NZ_CP021144:3695115-3695411 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT76048 NZ_CP021144:3695115-3695411 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References