Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2330949..2331603 Replicon chromosome
Accession NZ_CP021144
Organism Shigella sonnei strain 2015AM-1099

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag B9127_RS13040 Protein ID WP_000244777.1
Coordinates 2330949..2331356 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag B9127_RS13045 Protein ID WP_000354046.1
Coordinates 2331337..2331603 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9127_RS13020 (2326906) 2326906..2328639 - 1734 WP_000813167.1 single-stranded-DNA-specific exonuclease RecJ -
B9127_RS13025 (2328645) 2328645..2329355 - 711 WP_000715206.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
B9127_RS13030 (2329380) 2329380..2330276 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
B9127_RS13035 (2330388) 2330388..2330909 + 522 WP_001055874.1 flavodoxin FldB -
B9127_RS13040 (2330949) 2330949..2331356 - 408 WP_000244777.1 protein YgfX Toxin
B9127_RS13045 (2331337) 2331337..2331603 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
B9127_RS13050 (2331846) 2331846..2332826 + 981 WP_000886060.1 tRNA-modifying protein YgfZ -
B9127_RS13055 (2333022) 2333022..2333681 - 660 WP_000250274.1 hemolysin III family protein -
B9127_RS13060 (2333845) 2333845..2334156 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
B9127_RS13065 (2334201) 2334201..2335634 + 1434 WP_005137189.1 6-phospho-beta-glucosidase BglA -
B9127_RS13070 (2335691) 2335691..2336434 - 744 WP_000951937.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T76045 WP_000244777.1 NZ_CP021144:c2331356-2330949 [Shigella sonnei]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T76045 NZ_CP021144:c2331356-2330949 [Shigella sonnei]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT76045 WP_000354046.1 NZ_CP021144:c2331603-2331337 [Shigella sonnei]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT76045 NZ_CP021144:c2331603-2331337 [Shigella sonnei]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References