Detailed information of TA system TA076041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 776262..776746
• Replicon: chromosome
• Accession: NZ_CP021144
• Organism: Shigella sonnei strain 2015AM-1099

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A377EAU1
• Locus tag: B9127_RS04480
• Protein ID: WP_014334020.1
• Coordinates: 776262..776552 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A376K4R9
• Locus tag: B9127_RS04485
• Protein ID: WP_005142544.1
• Coordinates: 776552..776746 (-)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9127_RS04430 (771460)	771460..772536	-	1077	Protein_797	molybdopterin-dependent oxidoreductase	-
B9127_RS04440 (772594)	772594..773291	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
B9127_RS04445 (773285)	773285..774073	-	789	Protein_799	Rha family transcriptional regulator	-
B9127_RS04465 (774606)	774606..775303	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
B9127_RS04470 (775451)	775451..775816	-	366	WP_024174355.1	hypothetical protein	-
B9127_RS04475 (775828)	775828..775980	-	153	WP_000379558.1	DUF1391 family protein	-
B9127_RS04480 (776262)	776262..776552	-	291	WP_014334020.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B9127_RS04485 (776552)	776552..776746	-	195	WP_005142544.1	hypothetical protein	Antitoxin
B9127_RS04490 (776766)	776766..777266	-	501	WP_014334019.1	helix-turn-helix domain-containing protein	-
B9127_RS04495 (777378)	777378..777641	+	264	WP_001048456.1	helix-turn-helix transcriptional regulator	-
B9127_RS04500 (777638)	777638..778063	+	426	WP_000693826.1	toxin YdaT family protein	-
B9127_RS04505 (778135)	778135..779199	+	1065	WP_001262380.1	hypothetical protein	-
B9127_RS04510 (779206)	779206..779952	+	747	WP_000788740.1	ATP-binding protein	-
B9127_RS04515 (779974)	779974..780699	+	726	WP_000450631.1	DUF1627 domain-containing protein	-
B9127_RS04520 (780715)	780715..781137	+	423	WP_001151171.1	DUF977 family protein	-
B9127_RS04525 (781195)	781195..781551	+	357	WP_005140877.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	sodB / rhs/PAAR / rhs/PAAR / vgrG/tssI	683628..912183	228555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11062.93 Da
• Isoelectric Point: 10.2013

>T76041 WP_014334020.1 NZ_CP021144:c776552-776262 [Shigella sonnei]
MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLTEHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIE
VVNIVHSRRQYPGKNS

Nucleotide

• Length: 291 bp

>T76041 NZ_CP021144:c776552-776262 [Shigella sonnei]
ATGTTACCCGTTTTATGGCTACCATCTGCTCGTGATGATTTGCGCCAGATCGTAGCCTACATAGCTAAAGAAAATCCCGC
GGCAGCACGCAGAATGAAAATTCGAATTGAAACATCCGTTTTGCCGCTCACAGAGCATCCTTACCTGTATCCTCCAAGCG
AGCGAGTTCTTGGTCTTCGTGAAATTGTAACTCATCCTAACTACATCATCCTTTACCGAGTAACAGCATCAAGCATAGAA
GTAGTAAATATTGTGCATTCTCGAAGACAGTATCCAGGCAAAAACAGTTAA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7097.98 Da
• Isoelectric Point: 4.1759

>AT76041 WP_005142544.1 NZ_CP021144:c776746-776552 [Shigella sonnei]
MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVMAEMEQIIAQIAAATHRGE

Nucleotide

• Length: 195 bp

>AT76041 NZ_CP021144:c776746-776552 [Shigella sonnei]
ATGAGTACAGTCCTTTCCCCAATGGTTTCAGAATTTGAGACAGTTGAGCAAGAAAATAGTTACAACGAATGGCTGCGGGC
CAAAGTAGCAGCAAGCCTCGCGGATCCCCGCCCGGCGATTCCGCATGATGAGGTAATGGCGGAAATGGAACAAATTATTG
CCCAAATTGCTGCTGCAACTCACAGAGGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A377EAU1

Antitoxin

• AlphaFold DB: A0A376K4R9