Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 17923..18515 Replicon plasmid unnamed2
Accession NZ_CP021134
Organism Pseudomonas fragi strain NMC25

Toxin (Protein)


Gene name PumA Uniprot ID A0A266LNK0
Locus tag B6D87_RS23495 Protein ID WP_032901694.1
Coordinates 17923..18225 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A6I1WJJ7
Locus tag B6D87_RS23500 Protein ID WP_032901693.1
Coordinates 18222..18515 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6D87_RS23470 13554..15130 + 1577 Protein_11 PAS domain-containing methyl-accepting chemotaxis protein -
B6D87_RS23475 15370..15744 - 375 WP_157665720.1 hypothetical protein -
B6D87_RS23480 16027..16344 - 318 WP_086799818.1 hypothetical protein -
B6D87_RS23485 16398..16856 - 459 WP_086799819.1 hypothetical protein -
B6D87_RS23490 16912..17744 - 833 Protein_15 DUF945 domain-containing protein -
B6D87_RS23495 17923..18225 + 303 WP_032901694.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B6D87_RS23500 18222..18515 + 294 WP_032901693.1 putative addiction module antidote protein Antitoxin
B6D87_RS23505 19572..20492 + 921 WP_086799820.1 DUF2971 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..54359 54359


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(47-89)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11281.95 Da        Isoelectric Point: 9.5727

>T76029 WP_032901694.1 NZ_CP021134:17923-18225 [Pseudomonas fragi]
MKTIKQTATFMTWERKLKDHRAKAIIAARVFRVANGLLGDVEPVGQGVSELRIHYGPGYRVYFQQRGNELVLLLCGGDKS
SQARDIETAKMLASQWSEDE

Download         Length: 303 bp

>T76029 NZ_CP021134:17923-18225 [Pseudomonas fragi]
ATGAAAACGATCAAACAAACCGCCACGTTCATGACGTGGGAACGCAAGCTCAAGGATCACCGCGCAAAGGCGATCATTGC
GGCGCGGGTCTTCCGGGTAGCCAACGGCCTTTTGGGTGACGTTGAGCCGGTCGGTCAGGGCGTTAGCGAGTTGCGTATTC
ACTACGGCCCCGGCTACCGGGTCTATTTTCAGCAACGCGGCAATGAGCTTGTGCTGTTGTTGTGCGGCGGCGACAAAAGC
AGCCAGGCACGCGATATAGAAACCGCCAAGATGCTTGCCAGTCAGTGGAGTGAGGACGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10535.04 Da        Isoelectric Point: 5.6863

>AT76029 WP_032901693.1 NZ_CP021134:18222-18515 [Pseudomonas fragi]
MKEPIYEYDPAAALTDPESIAIFMSDAFETGDAGYIAKALGVVARAKGMTELAKESGLSREQLYRSFSDKGNPTLKTMLA
VMRAVGLDLTTRQHAHP

Download         Length: 294 bp

>AT76029 NZ_CP021134:18222-18515 [Pseudomonas fragi]
ATGAAAGAGCCAATCTATGAATATGATCCAGCGGCAGCGCTGACCGATCCTGAAAGCATCGCCATCTTTATGAGCGATGC
TTTTGAAACCGGCGACGCTGGCTACATTGCCAAAGCGCTAGGCGTTGTTGCCCGCGCCAAAGGCATGACAGAGCTGGCCA
AAGAATCCGGGTTGTCCCGTGAGCAGCTTTACCGCTCGTTCAGTGACAAGGGCAATCCAACCCTAAAAACCATGCTCGCC
GTCATGCGCGCTGTCGGCCTCGATCTGACAACCCGGCAACACGCACACCCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A266LNK0

Antitoxin

Source ID Structure
AlphaFold DB A0A6I1WJJ7

References