Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 4783601..4784228 Replicon chromosome
Accession NZ_CP021132
Organism Pseudomonas fragi strain NMC25

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag B6D87_RS22010 Protein ID WP_086799343.1
Coordinates 4784049..4784228 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag B6D87_RS22005 Protein ID WP_086799341.1
Coordinates 4783601..4784008 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6D87_RS21980 4778661..4779071 - 411 WP_016783077.1 type II toxin-antitoxin system HicB family antitoxin -
B6D87_RS21985 4779068..4779265 - 198 WP_081481088.1 type II toxin-antitoxin system HicA family toxin -
B6D87_RS21990 4779505..4780005 + 501 WP_086799334.1 sigma-70 family RNA polymerase sigma factor -
B6D87_RS21995 4780005..4780952 + 948 WP_086799336.1 FecR domain-containing protein -
B6D87_RS22000 4781065..4783503 + 2439 WP_086799338.1 TonB-dependent siderophore receptor -
B6D87_RS22005 4783601..4784008 - 408 WP_086799341.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B6D87_RS22010 4784049..4784228 - 180 WP_086799343.1 type II toxin-antitoxin system HicA family toxin Toxin
B6D87_RS22015 4784462..4785802 + 1341 WP_086799346.1 gluconate:H+ symporter -
B6D87_RS22020 4785846..4787192 + 1347 WP_086799348.1 D-serine ammonia-lyase -
B6D87_RS22025 4787276..4788223 + 948 WP_086799350.1 DNA-binding transcriptional regulator DsdC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6718.88 Da        Isoelectric Point: 10.9432

>T76028 WP_086799343.1 NZ_CP021132:c4784228-4784049 [Pseudomonas fragi]
MHSRQLITALEADGWYLVRSKGSHHHFKHPTKPGLVTIPHPKKDLLTKTINSILKQAQL

Download         Length: 180 bp

>T76028 NZ_CP021132:c4784228-4784049 [Pseudomonas fragi]
TTGCATAGCCGACAGCTGATAACTGCACTGGAAGCTGACGGTTGGTATCTGGTACGGAGTAAGGGAAGTCATCACCACTT
CAAGCACCCGACAAAGCCGGGGCTGGTAACGATTCCTCATCCGAAGAAAGATTTACTGACCAAAACGATCAACAGTATTT
TGAAGCAAGCTCAGCTTTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14715.79 Da        Isoelectric Point: 4.7465

>AT76028 WP_086799341.1 NZ_CP021132:c4784008-4783601 [Pseudomonas fragi]
MLYPIAIRPGDEQHAWGVEVPDIPGCFSAGENLDDAMAMAREAIEGHLEILAEDCSRIPVATPVSAHVANPEYGGCIWAL
VDIDITRYLGKAEKLNITLPGNLLNRIDEYVKHHPEQKSRSGFLASAALKVLQQA

Download         Length: 408 bp

>AT76028 NZ_CP021132:c4784008-4783601 [Pseudomonas fragi]
ATGCTTTACCCAATAGCCATTCGCCCTGGCGATGAGCAGCATGCCTGGGGTGTAGAGGTGCCGGATATTCCTGGGTGCTT
CTCTGCGGGGGAGAATCTTGACGACGCGATGGCCATGGCACGCGAAGCCATTGAAGGGCATCTGGAGATTTTGGCAGAGG
ATTGCAGCAGGATTCCTGTGGCAACCCCGGTAAGCGCTCATGTCGCTAACCCGGAGTATGGGGGCTGCATCTGGGCATTG
GTCGATATCGATATCACCAGATACCTCGGCAAGGCGGAAAAGCTGAATATTACCTTGCCCGGAAACTTGCTGAATCGGAT
CGATGAATATGTGAAACATCATCCTGAGCAAAAGAGCCGTTCAGGATTTCTGGCCTCGGCAGCATTGAAGGTTCTGCAAC
AAGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References