Detailed information of TA system TA076027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4778661..4779265
• Replicon: chromosome
• Accession: NZ_CP021132
• Organism: Pseudomonas fragi strain NMC25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: B6D87_RS21985
• Protein ID: WP_081481088.1
• Coordinates: 4779068..4779265 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A494R758
• Locus tag: B6D87_RS21980
• Protein ID: WP_016783077.1
• Coordinates: 4778661..4779071 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6D87_RS21955	4774131..4774805	+	675	WP_016783086.1	heavy metal response regulator transcription factor	-
B6D87_RS21960	4774798..4776162	+	1365	WP_086799330.1	heavy metal sensor histidine kinase	-
B6D87_RS21965	4776337..4777422	-	1086	WP_086799332.1	tartrate dehydrogenase	-
B6D87_RS21970	4777516..4778424	+	909	WP_029611679.1	LysR family transcriptional regulator	-
B6D87_RS21980	4778661..4779071	-	411	WP_016783077.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B6D87_RS21985	4779068..4779265	-	198	WP_081481088.1	type II toxin-antitoxin system HicA family toxin	Toxin
B6D87_RS21990	4779505..4780005	+	501	WP_086799334.1	sigma-70 family RNA polymerase sigma factor	-
B6D87_RS21995	4780005..4780952	+	948	WP_086799336.1	FecR domain-containing protein	-
B6D87_RS22000	4781065..4783503	+	2439	WP_086799338.1	TonB-dependent siderophore receptor	-
B6D87_RS22005	4783601..4784008	-	408	WP_086799341.1	type II toxin-antitoxin system HicB family antitoxin	-
B6D87_RS22010	4784049..4784228	-	180	WP_086799343.1	type II toxin-antitoxin system HicA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7226.44 Da
• Isoelectric Point: 11.0468

>T76027 WP_081481088.1 NZ_CP021132:c4779265-4779068 [Pseudomonas fragi]
MRSRELIELLMADGWFEIAVKGSHHQFKHPAKAGRVTVPHPRTEIAKGTLHSILKSAGLKGELRS

Nucleotide

• Length: 198 bp

>T76027 NZ_CP021132:c4779265-4779068 [Pseudomonas fragi]
ATGCGAAGTCGTGAACTTATCGAGTTGCTGATGGCGGATGGGTGGTTTGAGATCGCGGTTAAAGGAAGTCACCATCAGTT
CAAGCACCCAGCCAAAGCGGGTCGGGTAACGGTTCCGCATCCCAGGACAGAAATTGCCAAAGGGACGCTGCACAGCATTC
TCAAATCCGCAGGTCTCAAAGGAGAGTTACGGTCATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14844.63 Da
• Isoelectric Point: 4.4720

>AT76027 WP_016783077.1 NZ_CP021132:c4779071-4778661 [Pseudomonas fragi]
MKFPVVIHKDADSEFSVTVPDVPGCFSAGATFSQALDNVQEALALHLEGIVADGGELPQGKDVDAHLENPDYVGGIWAIV
DFDLTPYLGKSVRFNASLPENLLQRIDERVSRDHRYASRSGFLATAALRELSVQNF

Nucleotide

• Length: 411 bp

>AT76027 NZ_CP021132:c4779071-4778661 [Pseudomonas fragi]
ATGAAATTTCCAGTGGTAATACATAAAGACGCAGATTCTGAATTCAGCGTGACGGTGCCTGATGTGCCGGGCTGTTTTTC
TGCAGGGGCTACCTTTTCGCAAGCGCTGGATAACGTGCAGGAAGCATTGGCTTTGCATCTGGAAGGCATTGTTGCGGACG
GCGGTGAGCTACCCCAGGGCAAGGACGTGGACGCCCATCTGGAGAATCCGGATTATGTCGGCGGGATATGGGCAATCGTT
GACTTTGATTTGACGCCTTATCTCGGCAAGTCGGTTCGGTTCAACGCGTCTTTGCCAGAGAATCTGTTGCAGCGCATCGA
TGAACGAGTCAGCAGGGACCACCGCTATGCGTCCCGTTCAGGGTTTCTGGCAACAGCGGCATTGCGCGAGCTGAGCGTTC
AGAACTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A494R758