Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 235410..236047 Replicon plasmid pRetKim5d
Accession NZ_CP021128
Organism Rhizobium sp. Kim5

Toxin (Protein)


Gene name vapC Uniprot ID A0A060ICY2
Locus tag KIM5_RS31315 Protein ID WP_010059100.1
Coordinates 235646..236047 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name VapB1 Uniprot ID K0VQJ0
Locus tag KIM5_RS31310 Protein ID WP_008530479.1
Coordinates 235410..235646 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM5_RS31295 231404..232594 - 1191 WP_040112776.1 DUF1173 domain-containing protein -
KIM5_RS31300 232974..234011 + 1038 WP_086156711.1 toprim domain-containing protein -
KIM5_RS31305 234321..235259 + 939 WP_040112778.1 DUF2493 domain-containing protein -
KIM5_RS31310 235410..235646 + 237 WP_008530479.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KIM5_RS31315 235646..236047 + 402 WP_010059100.1 type II toxin-antitoxin system VapC family toxin Toxin
KIM5_RS31320 236184..236552 - 369 WP_086156712.1 WGR domain-containing protein -
KIM5_RS31325 236976..237299 + 324 WP_008530485.1 DUF736 family protein -
KIM5_RS34465 237614..237781 + 168 WP_164519071.1 hypothetical protein -
KIM5_RS31335 238106..238588 + 483 WP_009991774.1 hypothetical protein -
KIM5_RS31340 238661..240577 - 1917 WP_086156713.1 Ti-type conjugative transfer system protein TraG -
KIM5_RS31345 240568..240807 - 240 WP_009988310.1 conjugal transfer protein TraD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..684601 684601


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-117)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14935.23 Da        Isoelectric Point: 5.1972

>T76023 WP_010059100.1 NZ_CP021128:235646-236047 [Rhizobium sp. Kim5]
MPLYMLDTNMCIYLMKEQPPQVARRFAQCRVGDVVMSAITFAELEYGVSASTNAEKERANLEFLIEAIPVAPFDANAARA
YGPVRFATRERKKDHLDKLIAAHALSIDAVLVTNNVRDFSVYEGLPIENWLLS

Download         Length: 402 bp

>T76023 NZ_CP021128:235646-236047 [Rhizobium sp. Kim5]
ATGCCGCTTTATATGCTCGACACCAATATGTGTATCTACTTGATGAAGGAGCAGCCGCCGCAAGTCGCGCGTCGCTTTGC
TCAATGCCGTGTTGGCGACGTCGTCATGTCAGCCATCACATTTGCGGAACTTGAATATGGTGTCTCCGCATCGACGAACG
CGGAAAAGGAGCGGGCAAACCTGGAATTCCTGATAGAAGCGATTCCAGTTGCGCCCTTCGACGCCAATGCGGCGCGGGCC
TACGGGCCTGTCAGGTTTGCCACGCGGGAACGGAAAAAAGATCATCTGGACAAATTGATAGCCGCTCATGCGCTCTCGAT
TGATGCGGTCCTCGTAACTAACAATGTGCGGGACTTTTCCGTCTATGAAGGGTTGCCGATCGAGAACTGGTTGTTGTCGT
GA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8940.06 Da        Isoelectric Point: 5.1299

>AT76023 WP_008530479.1 NZ_CP021128:235410-235646 [Rhizobium sp. Kim5]
MHTTKVFKNGNSQAVRIPADLAYSQTEMDLEIERIGDELRIRPLKPSLEGVLSRFARFDRSFMAEGRGDQEQAERDTL

Download         Length: 237 bp

>AT76023 NZ_CP021128:235410-235646 [Rhizobium sp. Kim5]
ATGCACACGACCAAGGTGTTCAAAAACGGCAACTCACAAGCTGTACGCATCCCCGCGGATTTGGCCTACAGCCAGACCGA
GATGGACCTTGAAATCGAGCGGATCGGTGACGAGCTTCGCATCCGCCCGCTAAAGCCGTCCCTGGAAGGCGTGCTGTCGC
GTTTCGCAAGGTTTGACCGCTCGTTTATGGCGGAGGGACGCGGCGATCAGGAGCAGGCCGAGCGGGATACGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060ICY2

Antitoxin

Source ID Structure
AlphaFold DB K0VQJ0

References