Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 213348..214015 Replicon plasmid pRetKim5d
Accession NZ_CP021128
Organism Rhizobium sp. Kim5

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag KIM5_RS31185 Protein ID WP_077994590.1
Coordinates 213348..213791 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag KIM5_RS31190 Protein ID WP_183758872.1
Coordinates 213788..214015 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM5_RS31160 209100..210467 + 1368 WP_086156702.1 four-carbon acid sugar kinase family protein -
KIM5_RS31165 210537..211307 + 771 WP_077994581.1 3-oxoacyl-ACP reductase FabG -
KIM5_RS31170 211486..212496 + 1011 WP_077994582.1 2-keto-3-deoxygluconate permease -
KIM5_RS31175 212534..212851 + 318 WP_086156703.1 LysR family transcriptional regulator -
KIM5_RS31180 212896..213339 - 444 Protein_209 polyhydroxybutyrate depolymerase -
KIM5_RS31185 213348..213791 - 444 WP_077994590.1 PIN domain-containing protein Toxin
KIM5_RS31190 213788..214015 - 228 WP_183758872.1 CopG family transcriptional regulator Antitoxin
KIM5_RS31195 214216..214581 - 366 WP_077994592.1 MarR family transcriptional regulator -
KIM5_RS31200 214680..215219 + 540 WP_077994595.1 hypothetical protein -
KIM5_RS31205 215223..215882 + 660 WP_086156704.1 transglycosylase SLT domain-containing protein -
KIM5_RS31210 215879..216178 + 300 WP_009986199.1 TrbC/VirB2 family protein -
KIM5_RS31215 216184..216522 + 339 WP_009986201.1 type IV secretion system protein VirB3 -
KIM5_RS31220 216515..218881 + 2367 WP_086156705.1 VirB4 family type IV secretion system protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..684601 684601


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(2-131)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 15557.83 Da        Isoelectric Point: 7.4219

>T76022 WP_077994590.1 NZ_CP021128:c213791-213348 [Rhizobium sp. Kim5]
VTFLLDVNVLIALIDPGHVAHDDAHEWFAAIGQTAWATCPITENGVLRIVGNPKYPNSPGSPALVMEIVRKLRSLPGHSF
WPDDVSLVGSGDIAPTKILTPGQVTDTYLLALAKARGGQLATFDRKLSAAAVTRGNSALHPIATSRS

Download         Length: 444 bp

>T76022 NZ_CP021128:c213791-213348 [Rhizobium sp. Kim5]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTAATCGATCCGGGCCATGTAGCTCATGATGATGCGCACGAATG
GTTCGCTGCGATCGGTCAGACCGCGTGGGCTACCTGCCCCATTACCGAAAATGGTGTCCTCCGGATTGTCGGCAATCCCA
AATATCCCAACTCTCCCGGTTCACCGGCACTCGTGATGGAGATCGTCCGCAAATTGCGGTCACTCCCCGGTCACAGTTTT
TGGCCGGACGATGTCAGCCTTGTCGGGTCAGGCGATATCGCTCCCACGAAAATCCTAACACCGGGACAGGTAACTGACAC
CTACCTGCTTGCGCTAGCCAAGGCGCGTGGTGGCCAGCTGGCGACATTCGATCGCAAGCTGTCAGCGGCAGCGGTTACAA
GGGGCAATTCTGCCTTGCACCCGATCGCCACGAGCAGATCGTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8211.41 Da        Isoelectric Point: 9.1311

>AT76022 WP_183758872.1 NZ_CP021128:c214015-213788 [Rhizobium sp. Kim5]
MRTTLAIDDDVLIAAKAMATQQHRSVGEVISELARRSLRRPPSSGERNGIPLLSARPEAPPVTLEIVNALRDERP

Download         Length: 228 bp

>AT76022 NZ_CP021128:c214015-213788 [Rhizobium sp. Kim5]
ATGAGAACGACCTTGGCAATCGACGACGATGTCCTGATCGCCGCAAAAGCGATGGCAACCCAGCAGCACCGGAGCGTCGG
CGAGGTCATTTCTGAGCTGGCGAGACGTTCTCTGCGCCGACCGCCCAGCAGCGGTGAGCGCAACGGCATTCCTCTCCTGT
CAGCTCGGCCGGAAGCGCCGCCAGTGACGCTCGAAATCGTCAACGCCTTGCGTGACGAACGGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References