Detailed information of TA system TA076022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 213348..214015
• Replicon: plasmid pRetKim5d
• Accession: NZ_CP021128
• Organism: Rhizobium sp. Kim5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KIM5_RS31185
• Protein ID: WP_077994590.1
• Coordinates: 213348..213791 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KIM5_RS31190
• Protein ID: WP_183758872.1
• Coordinates: 213788..214015 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIM5_RS31160	209100..210467	+	1368	WP_086156702.1	four-carbon acid sugar kinase family protein	-
KIM5_RS31165	210537..211307	+	771	WP_077994581.1	3-oxoacyl-ACP reductase FabG	-
KIM5_RS31170	211486..212496	+	1011	WP_077994582.1	2-keto-3-deoxygluconate permease	-
KIM5_RS31175	212534..212851	+	318	WP_086156703.1	LysR family transcriptional regulator	-
KIM5_RS31180	212896..213339	-	444	Protein_209	polyhydroxybutyrate depolymerase	-
KIM5_RS31185	213348..213791	-	444	WP_077994590.1	PIN domain-containing protein	Toxin
KIM5_RS31190	213788..214015	-	228	WP_183758872.1	CopG family transcriptional regulator	Antitoxin
KIM5_RS31195	214216..214581	-	366	WP_077994592.1	MarR family transcriptional regulator	-
KIM5_RS31200	214680..215219	+	540	WP_077994595.1	hypothetical protein	-
KIM5_RS31205	215223..215882	+	660	WP_086156704.1	transglycosylase SLT domain-containing protein	-
KIM5_RS31210	215879..216178	+	300	WP_009986199.1	TrbC/VirB2 family protein	-
KIM5_RS31215	216184..216522	+	339	WP_009986201.1	type IV secretion system protein VirB3	-
KIM5_RS31220	216515..218881	+	2367	WP_086156705.1	VirB4 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..684601	684601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15557.83 Da
• Isoelectric Point: 7.4219

>T76022 WP_077994590.1 NZ_CP021128:c213791-213348 [Rhizobium sp. Kim5]
VTFLLDVNVLIALIDPGHVAHDDAHEWFAAIGQTAWATCPITENGVLRIVGNPKYPNSPGSPALVMEIVRKLRSLPGHSF
WPDDVSLVGSGDIAPTKILTPGQVTDTYLLALAKARGGQLATFDRKLSAAAVTRGNSALHPIATSRS

Nucleotide

• Length: 444 bp

>T76022 NZ_CP021128:c213791-213348 [Rhizobium sp. Kim5]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTAATCGATCCGGGCCATGTAGCTCATGATGATGCGCACGAATG
GTTCGCTGCGATCGGTCAGACCGCGTGGGCTACCTGCCCCATTACCGAAAATGGTGTCCTCCGGATTGTCGGCAATCCCA
AATATCCCAACTCTCCCGGTTCACCGGCACTCGTGATGGAGATCGTCCGCAAATTGCGGTCACTCCCCGGTCACAGTTTT
TGGCCGGACGATGTCAGCCTTGTCGGGTCAGGCGATATCGCTCCCACGAAAATCCTAACACCGGGACAGGTAACTGACAC
CTACCTGCTTGCGCTAGCCAAGGCGCGTGGTGGCCAGCTGGCGACATTCGATCGCAAGCTGTCAGCGGCAGCGGTTACAA
GGGGCAATTCTGCCTTGCACCCGATCGCCACGAGCAGATCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8211.41 Da
• Isoelectric Point: 9.1311

>AT76022 WP_183758872.1 NZ_CP021128:c214015-213788 [Rhizobium sp. Kim5]
MRTTLAIDDDVLIAAKAMATQQHRSVGEVISELARRSLRRPPSSGERNGIPLLSARPEAPPVTLEIVNALRDERP

Nucleotide

• Length: 228 bp

>AT76022 NZ_CP021128:c214015-213788 [Rhizobium sp. Kim5]
ATGAGAACGACCTTGGCAATCGACGACGATGTCCTGATCGCCGCAAAAGCGATGGCAACCCAGCAGCACCGGAGCGTCGG
CGAGGTCATTTCTGAGCTGGCGAGACGTTCTCTGCGCCGACCGCCCAGCAGCGGTGAGCGCAACGGCATTCCTCTCCTGT
CAGCTCGGCCGGAAGCGCCGCCAGTGACGCTCGAAATCGTCAACGCCTTGCGTGACGAACGGCCGTGA